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- Antigen structure
- References [4]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- 43-9200 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- NDUFS3 Monoclonal Antibody (17D95)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Positive control: Human heart mitochondria. In Flow Cytometry, this antibody gave a positive signal in methanol fixed/Tween permeabilised HepG2 cells.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 17D95
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references The transcriptional coregulator PGC-1β controls mitochondrial function and anti-oxidant defence in skeletal muscles.
Prevention and reversal of severe mitochondrial cardiomyopathy by gene therapy in a mouse model of Friedreich's ataxia.
The expression of nicotinamide N-methyltransferase increases ATP synthesis and protects SH-SY5Y neuroblastoma cells against the toxicity of Complex I inhibitors.
Identification of alternatively spliced GRIM-19 mRNA in kidney cancer tissues.
Gali Ramamoorthy T, Laverny G, Schlagowski AI, Zoll J, Messaddeq N, Bornert JM, Panza S, Ferry A, Geny B, Metzger D
Nature communications 2015 Dec 17;6:10210
Nature communications 2015 Dec 17;6:10210
Prevention and reversal of severe mitochondrial cardiomyopathy by gene therapy in a mouse model of Friedreich's ataxia.
Perdomini M, Belbellaa B, Monassier L, Reutenauer L, Messaddeq N, Cartier N, Crystal RG, Aubourg P, Puccio H
Nature medicine 2014 May;20(5):542-7
Nature medicine 2014 May;20(5):542-7
The expression of nicotinamide N-methyltransferase increases ATP synthesis and protects SH-SY5Y neuroblastoma cells against the toxicity of Complex I inhibitors.
Parsons RB, Aravindan S, Kadampeswaran A, Evans EA, Sandhu KK, Levy ER, Thomas MG, Austen BM, Ramsden DB
The Biochemical journal 2011 May 15;436(1):145-55
The Biochemical journal 2011 May 15;436(1):145-55
Identification of alternatively spliced GRIM-19 mRNA in kidney cancer tissues.
He X, Cao X
Journal of human genetics 2010 Aug;55(8):507-11
Journal of human genetics 2010 Aug;55(8):507-11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of NDUFS3 in isolated mitochondria and whole cell lysates using an NDUFS3 Monoclonal antibody (Product # 43-9200). The secondary antibody used was a Goat anti-Mouse IgG antibody. Lane 1: Isolated mitochondria from Human heart at 5 µg, Lane 2: Isolated mitochondria from Bovine heart at 4 µg, Lane 3: Isolated mitochondria from Rat heart at 10 µg, Lane 4: Isolated mitochondria from Mouse heart at 10 µg. Predicted band size: 30 kDa, Observed band size: 26 kDa. Extra bands in the mouse sample (lane 4) are due to the reaction of the IgG-specific goat anti-mouse secondary antibody with residual mouse blood in the heart tissue, as it is very difficult to entirely remove the blood from these small organs.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 Impact of myofibre PGC-1beta-deficiency on gene expression. ( a ) Pie chart of gene ontology annotation of genes downregulated by 1.2-fold in gastrocnemius of 8-week-old PGC-1beta (i)skm-/- mice compared with controls. ( b - d ) Relative transcript levels of genes encoding proteins involved in fatty-acid oxidation ( Acadm and Acadvl ; b ) in citric acid cycle ( Dlat , Fh1 , Mdh1 , Pdhb and Idh3b ) ( c ) and Oxidative phosphorylation (OXPHOS; Complex I: Ndufs3 , Ndufv1 , Ndufb3 , Ndufb7 ; Complex II: Sdhb , Sdhd ; Complex III: Cytc , Uqcrc1 , Uqcrfs1 , Uqcr11 ; Complex IV: Cox4i1 , Cox5b ; and Complex V: Atp5i , Atp5o ) ( d ) in gastrocnemius muscle of 8-week-old control and PGC-1beta (i)skm-/- mice, determined by RT-qPCR ( n =8). ( e ) Representative western blot analysis of Ndufs3 (complex I), Sdhb (complex II) and Cytc (complex III) from quadriceps muscle of 8, 18 and 26-week-old control and PGC-1beta (i)skm-/- mice (left panel), and average fold-change (right panel; n =6). Gapdh is used as the internal control. ( f ) Relative transcript levels of Tomm40l , Timm44 and Timm8a1 in gastrocnemius muscle of 8-week-old control and PGC-1beta (i)skm-/- mice, determined by RT-qPCR ( n =8). ( g ) Relative transcript levels of Mtg1 , Tsfm , Gfm1 , Mrpl18 , Mrpl47 , Mrpl55 and Mrps35 in gastrocnemius muscle of 8-week-old control and PGC-1beta (i)skm-/- mice, determined by RT-qPCR ( n =8). ( h ) Relative transcript levels of Mfn1 , Mfn2 , Opa1 , Drp1 and Fis1 in gastrocnemius m
