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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- P41-65R - Provider product page
- Provider
- SignalChem
- Product name
- Anti-phospho-PLK1 (Thr210)
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Purified by affinity chromatography
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian, Xenopus, Zebrafish
- Host
- Rabbit
- Epitope
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C (add 0.1% NaN3) for several months, and at -20°C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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Supportive validation
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- SignalChem (provider)
- Main image
- Experimental details
- Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK1 protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PLK1(Thr210) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
- Sample type
- Western Blot
