Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Proximity ligation assay [1]
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Validation data
Reference
Comment
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- Product number
- H00005347-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005347-M01, RRID:AB_425597
- Product name
- PLK1 monoclonal antibody (M01), clone 2G12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant PLK1.
- Antigen sequence
MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAP
PAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEI
SDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIH
RSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLE
LHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHR
DLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKT
LCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLL
VGKPPFETSCLKETYLRIKKNEYSIPKHINPVAAS
LIQKMLQTDPTARPTINELLNDEFFTSGYIPARLP
ITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENP
LPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVN
ASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDK
YGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIE
RDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHL
LKAGANITPREGDELARLPYLRTWFRTRSAIILHL
SNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFR
TYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRS
ASNRLKAS- Isotype
- IgG
- Antibody clone number
- 2G12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PLK1 monoclonal antibody (M01), clone 2G12 Western Blot analysis of PLK1 expression in Hela S3 NE ( Cat # L013V3 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using anti-PLK1 monoclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody (M01), clone 2G12.Lane 1: PLK1 transfected lysate(68.3 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged PLK1 is approximately 1ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PLK1 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CCNB1 and PLK1. Mahlavu cells were stained with anti-CCNB1 rabbit purified polyclonal 1:1200 and anti-PLK1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)