PAB28261

antibody from Abnova Corporation

Targeting: RMDN1 CGI-90, FAM82B, FLJ20665, RMD1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28261

Provider

Abnova Corporation

Product name

FAM82B polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant FAM82B.

Antigen sequence

RLARASRDVAQLSRTSEEEKKLLVYEALEYAKRAL
EKNESSFASHKWYAICLSDVGDYEGIKAKIANAYI
IKEHFEKAIELNPKDATSIHL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells with FAM82B polyclonal antibody (Cat # PAB28261) at 1:100 - 1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with FAM82B polyclonal antibody (Cat # PAB28261) at 1-4 ug/ml shows positivity in centrosome.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human heart muscle with FAM82B polyclonal antibody (Cat # PAB28261) shows distinct positivity in intercalated discs at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)