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Western blot
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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB7322 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7322, RRID:AB_1574078
- Product name
- COX4I1/COX4I2 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to human COX4I1/COX4I2.
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-QGLASKWDYEKNE- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references HIF-1 regulates cytochrome oxidase subunits to optimize efficiency of respiration in hypoxic cells.
Fukuda R, Zhang H, Kim JW, Shimoda L, Dang CV, Semenza GL
Cell 2007 Apr 6;129(1):111-22
Cell 2007 Apr 6;129(1):111-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- COX4I1/COX4I2 polyclonal antibody (Cat # PAB7322) (0.01 ug/mL) staining of human muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- COX4I1/COX4I2 polyclonal antibody (Cat # PAB7322, 2.5 ug/mL) staining of paraffin embedded human thyroid gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
