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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- GTX101499 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101499, RRID:AB_1950020
- Product name
- COX4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references ATM mediates spermidine-induced mitophagy via PINK1 and Parkin regulation in human fibroblasts.
RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Qi Y, Qiu Q, Gu X, Tian Y, Zhang Y
Scientific reports 2016 Apr 19;6:24700
Scientific reports 2016 Apr 19;6:24700
RRM2B-Mediated Regulation of Mitochondrial Activity and Inflammation under Oxidative Stress.
Cho EC, Kuo ML, Cheng JH, Cheng YC, Hsieh YC, Liu YR, Hsieh RH, Yen Y
Mediators of inflammation 2015;2015:287345
Mediators of inflammation 2015;2015:287345
Synergistic protection of N-acetylcysteine and ascorbic acid 2-phosphate on human mesenchymal stem cells against mitoptosis, necroptosis and apoptosis.
Li CJ, Sun LY, Pang CY
Scientific reports 2015 Apr 24;5:9819
Scientific reports 2015 Apr 24;5:9819
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g of whole cell lysate)A:A431(GTX27909) B:MOLT4(GTX27912)12% SDS PAGEGTX101499 diluted at 1:2000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 15% SDS-PAGE, and the membrane was blotted with COX4 antibody (GTX101499) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using COX4(GTX101499) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of COX4 protein from 293T whole cell extracts using 5 £gg of COX4 antibody (GTX101499).Western blot analysis was performed using COX4 antibody (GTX101499).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using COX4(GTX101499) antibody at 1:100 dilution.
