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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NB110-39115 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB110-39115, RRID:AB_2085715
- Product name
- Rabbit Polyclonal COX4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Bovine, Drosophila, Porcine, Simian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references ROCK inhibitors upregulate the neuroprotective Parkin-mediated mitophagy pathway.
Inhibition of Alternative Cancer Cell Metabolism of EGFR Mutated Non-Small Cell Lung Cancer Serves as a Potential Therapeutic Strategy.
Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.
Shear stress and oxygen availability drive differential changes in opossum kidney proximal tubule cell metabolism and endocytosis.
Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1.
High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury.
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.
Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions.
Moskal N, Riccio V, Bashkurov M, Taddese R, Datti A, Lewis PN, Angus McQuibban G
Nature communications 2020 Jan 3;11(1):88
Nature communications 2020 Jan 3;11(1):88
Inhibition of Alternative Cancer Cell Metabolism of EGFR Mutated Non-Small Cell Lung Cancer Serves as a Potential Therapeutic Strategy.
Huang CY, Hsu LH, Chen CY, Chang GC, Chang HW, Hung YM, Liu KJ, Kao SH
Cancers 2020 Jan 10;12(1)
Cancers 2020 Jan 10;12(1)
Mapping Interactome Networks of DNAJC11, a Novel Mitochondrial Protein Causing Neuromuscular Pathology in Mice.
Violitzi F, Perivolidi VI, Thireou T, Grivas I, Haralambous S, Samiotaki M, Panayotou G, Douni E
Journal of proteome research 2019 Nov 1;18(11):3896-3912
Journal of proteome research 2019 Nov 1;18(11):3896-3912
Shear stress and oxygen availability drive differential changes in opossum kidney proximal tubule cell metabolism and endocytosis.
Ren Q, Gliozzi ML, Rittenhouse NL, Edmunds LR, Rbaibi Y, Locker JD, Poholek AC, Jurczak MJ, Baty CJ, Weisz OA
Traffic (Copenhagen, Denmark) 2019 Jun;20(6):448-459
Traffic (Copenhagen, Denmark) 2019 Jun;20(6):448-459
Necroptosis in pulmonary macrophages mediates lipopolysaccharide-induced lung inflammatory injury by activating ZBP-1.
Du XK, Ge WY, Jing R, Pan LH
International immunopharmacology 2019 Dec;77:105944
International immunopharmacology 2019 Dec;77:105944
High Tidal Volume Induces Mitochondria Damage and Releases Mitochondrial DNA to Aggravate the Ventilator-Induced Lung Injury.
Lin JY, Jing R, Lin F, Ge WY, Dai HJ, Pan L
Frontiers in immunology 2018;9:1477
Frontiers in immunology 2018;9:1477
Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
Long KR, Shipman KE, Rbaibi Y, Menshikova EV, Ritov VB, Eshbach ML, Jiang Y, Jackson EK, Baty CJ, Weisz OA
Molecular biology of the cell 2017 Sep 15;28(19):2508-2517
Molecular biology of the cell 2017 Sep 15;28(19):2508-2517
Differential regulation of HIF-mediated pathways increases mitochondrial metabolism and ATP production in hypoxic osteoclasts.
Morten KJ, Badder L, Knowles HJ
The Journal of pathology 2013 Apr;229(5):755-64
The Journal of pathology 2013 Apr;229(5):755-64
Endonuclease G plays a role in immunoglobulin class switch DNA recombination by introducing double-strand breaks in switch regions.
Zan H, Zhang J, Al-Qahtani A, Pone EJ, White CA, Lee D, Yel L, Mai T, Casali P
Molecular immunology 2011 Jan;48(4):610-22
Molecular immunology 2011 Jan;48(4):610-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: COX4 Antibody [NB110-39115] - Image shows a specific band for COX4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NB110-39115] - Analysis of COX4 in porcine whole skeletal muscle lysate using anti-COX4 antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NB110-39115] - Analysis of COX4 in the following cell lysates: 1. HeLa, 2. Ntera, 3. A431, 4. HepG2, 5. MCF7 and 6. 3T3.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NB110-39115] - Detection of COX4-1 isoform using COX4 antibody NB110-39115. Lane 1: HEK 293 with empty vector, Lane 2: HEK 293 with flag-tagged human COX4-1, Lane 3: HEK 293 with flag-tagged human COX4-2. Photo courtesy of Ryo Fukuda, G. Semenza lab. Johns Hopkins, SOM
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: COX4 Antibody [NB110-39115] - Total protein from HeLa, 3T3 and PC12 was separated on a 4-20% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-COX4 in 5% block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: COX4 Antibody [NB110-39115] - Analysis of COX4 in human breast cancer using DAB with hematoxylin counterstain.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: COX4 Antibody [NB110-39115] - Analysis of methanol fixed drosophila embryo using 1:200 dilution of COX4 antibody. The signal was developed using AF488 conjugated Donkey anti-Rabbit IgG (H+L) secondary antibody and the sections were further counterstained for tubulin and DAPI. The antibody generated a specific staining of COX4 in mitochondria near mitotic spindles at early stage of embryo development. Image from verified customer review.
