AMAb91173

antibody from Atlas Antibodies

Targeting: COX4I1 COX4, COX4-1

Western blot (Enhanced validation)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91173

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91173, RRID:AB_2665833

Product name

Anti-COX4I1

Antibody type

Monoclonal

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

LATRVFSLVGKRAISTSVCVRAHESVVKSEDFSLP
AYMDRRDHPLPEVAHVKHLSASQKALKEKEKASWS
SLSMDEKVELYRIKFKESFAEMNRGSNEWKT


Epitope

Binds to an epitope located within the peptide sequence EKVELYRIKFKESFA as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL3501

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-COX4I1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431, human cell line HepG2 and mouse cell line NIH-3T3.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-COX4I1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human fallopian tube shows strong cytoplasmic immunoreactivity in epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in renal tubules cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human stomach shows strong cytoplasmic immunoreactivity in glandular epithelium cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows cytoplasmic positivity in the epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows cytoplasmic immunoreactivity in hepatocytes.