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- References [4]
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- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP1-00161 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00161, RRID:AB_1502734
- Product name
- Goat Polyclonal BHMT Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Hepatic metabolite profiles in mice with a suboptimal selenium status.
Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels.
Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism.
Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice.
Geillinger KE, Rathmann D, Köhrle J, Fiamoncini J, Daniel H, Kipp AP
The Journal of nutritional biochemistry 2014 Sep;25(9):914-22
The Journal of nutritional biochemistry 2014 Sep;25(9):914-22
Methyl-donor supplementation in obese mice prevents the progression of NAFLD, activates AMPK and decreases acyl-carnitine levels.
Dahlhoff C, Worsch S, Sailer M, Hummel BA, Fiamoncini J, Uebel K, Obeid R, Scherling C, Geisel J, Bader BL, Daniel H
Molecular metabolism 2014 Aug;3(5):565-80
Molecular metabolism 2014 Aug;3(5):565-80
Hepatic methionine homeostasis is conserved in C57BL/6N mice on high-fat diet despite major changes in hepatic one-carbon metabolism.
Dahlhoff C, Desmarchelier C, Sailer M, Fürst RW, Haag A, Ulbrich SE, Hummel B, Obeid R, Geisel J, Bader BL, Daniel H
PloS one 2013;8(3):e57387
PloS one 2013;8(3):e57387
Inhibition of betaine-homocysteine S-methyltransferase causes hyperhomocysteinemia in mice.
Collinsova M, Strakova J, Jiracek J, Garrow TA
The Journal of nutrition 2006 Jun;136(6):1493-7
The Journal of nutrition 2006 Jun;136(6):1493-7
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BHMT Antibody [NBP1-00161] - Rat Liver lysate (35 ug protein in RIPA buffer). Antibody at 0.03 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BHMT Antibody [NBP1-00161] - HEK293 overexpressing BHMT and probed with this antibody (mock transfection in first lane).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Liver tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: BHMT Antibody [NBP1-00161] - Human Kidney tissue. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
