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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- MA5-34901 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BHMT Monoclonal Antibody (9E1)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: Human kidney tissue lysate, human liver tissue, HepG2, SiHa, rat kidney tissue, mouse liver tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 9E1
- Vial size
- 100 µL
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of BHMT in human liver tissue and human kidney tissue lysate. Samples were incubated with BHMT monoclonal antibody (Product # MA5-34901), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BHMT in SiHa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with BHMT monoclonal antibody (Product # MA5-34901), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BHMT in HepG2 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with BHMT monoclonal antibody (Product # MA5-34901), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BHMT in paraffin-embedded rat kidney tissue. Samples were incubated with BHMT monoclonal antibody (Product # MA5-34901), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BHMT in paraffin-embedded mouse liver tissue. Samples were incubated with BHMT monoclonal antibody (Product # MA5-34901), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of BHMT in paraffin-embedded human liver tissue. Samples were incubated with BHMT monoclonal antibody (Product # MA5-34901), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of BHMT in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with BHMT monoclonal antibody (Product # MA5-34901) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-mouse IgG1.
