Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-30204 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UQCRC2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Jurkat, Raji, NCI-H929, mouse brain, rat brain.
- Concentration
- 1 mg/mL
Submitted references Absence of TXNIP in Humans Leads to Lactic Acidosis and Low Serum Methionine Linked to Deficient Respiration on Pyruvate.
Nuclear respiratory factor-1 and bioenergetics in tamoxifen-resistant breast cancer cells.
Katsu-Jiménez Y, Vázquez-Calvo C, Maffezzini C, Halldin M, Peng X, Freyer C, Wredenberg A, Giménez-Cassina A, Wedell A, Arnér ESJ
Diabetes 2019 Apr;68(4):709-723
Diabetes 2019 Apr;68(4):709-723
Nuclear respiratory factor-1 and bioenergetics in tamoxifen-resistant breast cancer cells.
Radde BN, Ivanova MM, Mai HX, Alizadeh-Rad N, Piell K, Van Hoose P, Cole MP, Muluhngwi P, Kalbfleisch TS, Rouchka EC, Hill BG, Klinge CM
Experimental cell research 2016 Sep 10;347(1):222-231
Experimental cell research 2016 Sep 10;347(1):222-231
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UQCRC2 using 30 µg of Jurkat lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a UQCRC2 polyclonal antibody (Product # PA5-30204) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UQCRC2 was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a UQCRC2 Polyclonal Antibody (Product # PA5-30204) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of HepG2 (Lane 1), CaCo2 (Lane 2), A-431 (Lane 3), Jurkat (Lane 4), U937 untreated (Lane 5) or treated with LPS (100 ng/mL for 24 hours) (Lane 6). The blot was probed with Anti-UQCRC2 Polyclonal Antibody (Product # PA5-30204, 1:2500 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 48 kDa band corresponding to UQCRC2 was observed in all the cell lines tested and was observed to be induced upon LPS treatment of U937 cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UQCRC2 in paraformaldehyde-fixed A431 cells using a UQCRC2 polyclonal antibody (Product # PA5-30204) at a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of UQCRC2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with UQCRC2 Rabbit Polyclonal Antibody (Product # PA5-30204) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UQCRC2 Polyclonal Antibody detects UQCRC2 protein at cytoplasm on human breast cancer by immunohistochemical analysis. Sample: Paraffin-embedded breast cancer. UQCRC2 Polyclonal Antibody (Product # PA5-30204) dilution: 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- UQCRC2 Polyclonal Antibody immunoprecipitates UQCRC2 protein in IP experiments. IP samples: HepG2 whole cell extract. A. 35 µg HepG2 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of UQCRC2 protein by 4 µg UQCRC2 Polyclonal Antibody (Product # PA5-30204). 10 % SDS-PAGE. The immunoprecipitated UQCRC2 protein was detected by UQCRC2 Polyclonal Antibody (Product # PA5-30204) diluted at 1:5000.
