MA5-11538

antibody from Invitrogen Antibodies

Targeting: GNRHR GRHR, LHRHR

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-11538

Provider

Invitrogen Antibodies

Product name

GnRHR Monoclonal Antibody (GNRH03)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-11538 targets GnRH Receptor in FACS, IF, and IHC (P) applications and shows reactivity with Human samples.

Antibody clone number

GNRH03

Concentration

0.2 mg/mL

References

Submitted references

GnRH antagonist treatment of malignant adrenocortical tumors.

Doroszko M, Chrusciel M, Stelmaszewska J, Slezak T, Anisimowicz S, Plöckinger U, Quinkler M, Bonomi M, Wolczynski S, Huhtaniemi I, Toppari J, Rahman NA
Endocrine-related cancer 2019 Jan 1;26(1):103-117

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In search of the molecular mechanisms mediating the inhibitory effect of the GnRH antagonist degarelix on human prostate cell growth.

Sakai M, Martinez-Arguelles DB, Patterson NH, Chaurand P, Papadopoulos V
PloS one 2015;10(3):e0120670

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Emerging association between androgen deprivation therapy and male meningioma: significant expression of luteinizing hormone-releasing hormone receptor in male meningioma.

Li Q, Coulson H, Klaassen Z, Sharma S, Ramalingam P, Moses KA, Terris MK
Prostate cancer and prostatic diseases 2013 Dec;16(4):387-90

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Gonadotropin-releasing hormone-II increases membrane type I metalloproteinase production via beta-catenin signaling in ovarian cancer cells.

Ling Poon S, Lau MT, Hammond GL, Leung PC
Endocrinology 2011 Mar;152(3):764-72

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Effects of gonadotrophin releasing hormone analogues on human endometrial stromal cells and embryo invasion in vitro.

Klemmt PA, Liu F, Carver JG, Jones C, Brosi D, Adamson J, Mardon HJ, McVeigh E
Human reproduction (Oxford, England) 2009 Sep;24(9):2187-92

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GnRH receptor expression in human prostate cancer cells is affected by hormones and growth factors.

Angelucci C, Lama G, Iacopino F, Ferracuti S, Bono AV, Millar RP, Sica G
Endocrine 2009 Aug;36(1):87-97

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Presence of immunoreactive gonadotropin releasing hormone (GnRH) and its receptor (GnRHR) in rat ovary during pregnancy.

Sengupta A, Chakrabarti N, Sridaran R
Molecular reproduction and development 2008 Jun;75(6):1031-44

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Expression and localization of gonadotropin-releasing hormone receptor in the rat oviduct during pregnancy.

Sengupta A, Sridaran R
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2008 Jan;56(1):25-31

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed, paraffin-embedded human pituitary stained with GnRH-Receptor antibody using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of anterior pituitary cells.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5 GNRHR knockdown in H295R adrenocortical carcinoma cells. Western blot analysis of GNRHR expression in WT (H295R), transfected with non-targeting (siControl) or GNRHR-targeting (siGNRHR) siRNA H295R adrenocortical carcinoma cells (A). Densitometric analysis of the GNRHR protein levels in H295R, siControl and siGNRHR cells ( n = 3) (B). The effect of CTX treatment on proliferation ( n = 12) (C) and caspase 3/7 activation ( n = 9) (D) in siControl and siGNRHR cells (mean +- s.e.m. ; * P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 3 GnRHR levels in BPH-1 and LNCaP cells. Representative immunoblots of BPH-1 and LNCaP cell extracts. Each lane represents an independent cell passage (n = 3).