HPA064892

antibody from Atlas Antibodies

Targeting: SEMG1 CT103, SEMG

Antibody data

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Validation data

Product number: HPA064892 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA064892, RRID:AB_2732724
Product name: Anti-SEMG1
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: APNPKQEPWHGENAKGESGQSTNRE
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

SEMG1 protein structure - HPA064892 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
Main image
Experimental details: Immunohistochemistry analysis in human seminal vesicle and lymph node tissues using Anti-SEMG1 antibody. Corresponding SEMG1 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
Main image
Experimental details: Immunohistochemical staining of human colon, kidney, liver and seminal vesicle using Anti-SEMG1 antibody HPA064892 (A) shows similar protein distribution across tissues to independent antibody HPA042476 (B).
Antibody #2 product nr: HPA042476
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human seminal vesicle shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human lymph node shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-SEMG1 antibody HPA064892.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-SEMG1 antibody HPA064892.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human colon using Anti-SEMG1 antibody HPA064892.
Sample type: HUMAN