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Western blotAntibody data
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- Antigen structure
- References [6]
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- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX101943 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX101943, RRID:AB_1240487
- Product name
- ATF4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Neurite atrophy and apoptosis mediated by PERK signaling after accumulation of GM2-ganglioside.
Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.
RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK-CHOP signaling.
Luteolin modulates 6-hydroxydopamine-induced transcriptional changes of stress response pathways in PC12 cells.
Cab45S inhibits the ER stress-induced IRE1-JNK pathway and apoptosis via GRP78/BiP.
Functional RNA interference (RNAi) screen identifies system A neutral amino acid transporter 2 (SNAT2) as a mediator of arsenic-induced endoplasmic reticulum stress.
Virgolini MJ, Feliziani C, Cambiasso MJ, Lopez PH, Bollo M
Biochimica et biophysica acta. Molecular cell research 2019 Feb;1866(2):225-239
Biochimica et biophysica acta. Molecular cell research 2019 Feb;1866(2):225-239
Endoplasmic Reticulum Protein TXNDC5 Augments Myocardial Fibrosis by Facilitating Extracellular Matrix Protein Folding and Redox-Sensitive Cardiac Fibroblast Activation.
Shih YC, Chen CL, Zhang Y, Mellor RL, Kanter EM, Fang Y, Wang HC, Hung CT, Nong JY, Chen HJ, Lee TH, Tseng YS, Chen CN, Wu CC, Lin SL, Yamada KA, Nerbonne JM, Yang KC
Circulation research 2018 Apr 13;122(8):1052-1068
Circulation research 2018 Apr 13;122(8):1052-1068
RCN1 suppresses ER stress-induced apoptosis via calcium homeostasis and PERK-CHOP signaling.
Xu S, Xu Y, Chen L, Fang Q, Song S, Chen J, Teng J
Oncogenesis 2017 Mar 20;6(3):e304
Oncogenesis 2017 Mar 20;6(3):e304
Luteolin modulates 6-hydroxydopamine-induced transcriptional changes of stress response pathways in PC12 cells.
Hu LW, Yen JH, Shen YT, Wu KY, Wu MJ
PloS one 2014;9(5):e97880
PloS one 2014;9(5):e97880
Cab45S inhibits the ER stress-induced IRE1-JNK pathway and apoptosis via GRP78/BiP.
Chen L, Xu S, Liu L, Wen X, Xu Y, Chen J, Teng J
Cell death & disease 2014 May 8;5:e1219
Cell death & disease 2014 May 8;5:e1219
Functional RNA interference (RNAi) screen identifies system A neutral amino acid transporter 2 (SNAT2) as a mediator of arsenic-induced endoplasmic reticulum stress.
Oh RS, Pan WC, Yalcin A, Zhang H, Guilarte TR, Hotamisligil GS, Christiani DC, Lu Q
The Journal of biological chemistry 2012 Feb 17;287(8):6025-34
The Journal of biological chemistry 2012 Feb 17;287(8):6025-34
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa (control)? B: HeLa treat tunicamycin (4ug/ml) 12% SDS PAGE GTX101943 diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HeLa whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with ATF4 antibody (GTX101943) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ATF4 antibody detects ATF4 protein at cytoplasm and nucleus by immunofluorescent analysis. Samples: HeLa cells mock (left) and treated with 2 £gg/ml Tunicamycin for 8hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATF4 protein stained by ATF4 antibody (GTX101943) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
