LS-C782141

antibody from LSBio

Targeting: ATF4 CREB-2, TAXREB67, TXREB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C782141

Provider

LSBio

Product name

ATF4 Antibody LS-C782141

Antibody type

Polyclonal

Description

Antigen Affinity purification

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

After reconstitution, store at 4°C for up to 1 month. Long-term: aliquot and store at -20°C. Avoid freeze-thaws cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa, 2) placenta, 3) HepG2, 4) MDA-MB-231 and 5) SW620 lysate with ATF4 antibody at 0.5ug/ml. Predicted molecular weight ~39 kDa, observed here at ~49 kDa.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE human prostate cancer with ATF4 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE human ovarian cancer with ATF4 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC testing of FFPE mouse small intestine with ATF4 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.