10835-1-AP

antibody from Invitrogen Antibodies

Targeting: ATF4 CREB-2, TAXREB67, TXREB

Antibody data

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Validation data

Product number: 10835-1-AP - Provider product page
Provider: Invitrogen Antibodies
Product name: ATF4 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Other
Description: This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated forms (50 kDa). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.
Concentration: 0.27 mg/mL

ATF4 protein structure - 10835-1-AP shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: SGC-7901 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: A431 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: DU 145 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Rat brain tissue were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.