NBP1-28751

antibody from Novus Biologicals

Targeting: TNRC6A CAGH26, GW182, KIAA1460, TNRC6

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

NBP1-28751

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-28751, RRID:AB_1913954

Product name

Rabbit Polyclonal GW182 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR.

Wilczynska A, Gillen SL, Schmidt T, Meijer HA, Jukes-Jones R, Langlais C, Kopra K, Lu WT, Godfrey JD, Hawley BR, Hodge K, Zanivan S, Cain K, Le Quesne J, Bushell M
Genome biology 2019 Dec 2;20(1):262

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Reassessment of Exosome Composition.

Jeppesen DK, Fenix AM, Franklin JL, Higginbotham JN, Zhang Q, Zimmerman LJ, Liebler DC, Ping J, Liu Q, Evans R, Fissell WH, Patton JG, Rome LH, Burnette DT, Coffey RJ
Cell 2019 Apr 4;177(2):428-445.e18

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Crosstalk between alternative polyadenylation and miRNAs in the regulation of protein translational efficiency.

Fu Y, Chen L, Chen C, Ge Y, Kang M, Song Z, Li J, Feng Y, Huo Z, He G, Hou M, Chen S, Xu A
Genome research 2018 Nov;28(11):1656-1663

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MicroRNAs of miR-17-92 cluster increase gene expression by targeting mRNA-destabilization pathways.

Jung E, Seong Y, Jeon B, Kwon YS, Song H
Biochimica et biophysica acta. Gene regulatory mechanisms 2018 Jun 20;1861(7):603-612

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MicroRNAs recruit eIF4E2 to repress translation of target mRNAs.

Chen S, Gao G
Protein & cell 2017 Oct;8(10):750-761

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CK2 targeted RNAi therapeutic delivered via malignant cell-directed tenfibgen nanocapsule: dose and molecular mechanisms of response in xenograft prostate tumors.

Ahmed K, Kren BT, Abedin MJ, Vogel RI, Shaughnessy DP, Nacusi L, Korman VL, Li Y, Dehm SM, Zimmerman CL, Niehans GA, Unger GM, Trembley JH
Oncotarget 2016 Sep 20;7(38):61789-61805

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Human 4E-T represses translation of bound mRNAs and enhances microRNA-mediated silencing.

Kamenska A, Lu WT, Kubacka D, Broomhead H, Minshall N, Bushell M, Standart N
Nucleic acids research 2014 Mar;42(5):3298-313

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Translational repression and eIF4A2 activity are critical for microRNA-mediated gene regulation.

Meijer HA, Kong YW, Lu WT, Wilczynska A, Spriggs RV, Robinson SW, Godfrey JD, Willis AE, Bushell M
Science (New York, N.Y.) 2013 Apr 5;340(6128):82-5

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Microprocessor, Setx, Xrn2, and Rrp6 co-operate to induce premature termination of transcription by RNAPII.

Wagschal A, Rousset E, Basavarajaiah P, Contreras X, Harwig A, Laurent-Chabalier S, Nakamura M, Chen X, Zhang K, Meziane O, Boyer F, Parrinello H, Berkhout B, Terzian C, Benkirane M, Kiernan R
Cell 2012 Sep 14;150(6):1147-57

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GW182 Antibody [NBP1-28751] - Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 10 ug/mg lysate. GW182 was also immunoprecipitated by rabbit anti-GW182 antibody NBP1-28702, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: GW182 Antibody [NBP1-28751] - Detection of Human GW182 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-GW182 antibody NBP1-28751 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.