CF503193

antibody from Invitrogen Antibodies

Targeting: LGR4 GPR48

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

CF503193

Provider

Invitrogen Antibodies

Product name

LGR4 Monoclonal Antibody (OTI2D1), TrueMAB™

Antibody type

Monoclonal

Antigen

Recombinant protein fragment

Description

For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2D1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LGR4 (RC221345, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-LGR4. Positive lysates LY413017 (100 µg) and LC413017 (20 µg) can be purchased separately from OriGene.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK293T cells transfected with either RC221345 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-LGR4 antibody (TA503193), and then analyzed by flow cytometry.