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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- ARP40485_P050 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#ARP40485_P050, RRID:AB_2045707
- Product name
- EIF3G antibody - middle region (ARP40485_P050)
- Antibody type
- Polyclonal
- Antigen
- The immunogen is a synthetic peptide directed towards the middle region of human EIF3G
- Description
- Affinity Purified
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Goat, Guinea Pig, Horse, Zebrafish
- Host
- Rabbit
- Vial size
- 100 μl
- Concentration
- Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- Storage
- For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis using Anti-EIF3G antibody detects protein at predicted molecular weight using Jurkat Cells as a positive control. Tissue/Lysate Concentration: 1ug/ml Antibody Concentration: 1.0ug/ml
- Sample type
- Jurkat Cells
- Validation comment
- Western blot analysis detects protein at predicted molecular weight
- Primary Ab dilution
- 1.0ug/ml
- Other comments
- Lysate concentration 1ug/ml
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Amount and Sample Type: 500µg mouse brain homogenateAmount of IP Antibody: 6µg Primary Antibody: EIF3GPrimary Antibody Dilution: 1:500 Secondary Antibody: Goat anti-rabbit Alexa-Fluor 594 Secondary Antibody Dilution: 1:5000Gene Name: EIF3GSubmitted by: Dr. Yuzhi Chen, University of Arkansas for Medical Science
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Sample Type : Human brain stem cellsPrimary Antibody Dilution : 1:500Secondary Antibody : Goat anti-rabbit Alexa-Fluor 594Secondary Antibody Dilution : 1:1000Color/Signal Descriptions : EIF3G: Red DAPI:BlueGene Name : EIF3GSubmitted by : Dr. Yuzhi Chen, University of Arkansas for Medical Science
