Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PAB0176 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0176, RRID:AB_1018941
- Product name
- CCR1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CCR1.
- Antigen sequence
VSSTSPSTGEHE
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Extracellular ATP mediates mast cell-dependent intestinal inflammation through P2X7 purinoceptors.
CCR1/CCL5 (RANTES) receptor-ligand interactions modulate allogeneic T-cell responses and graft-versus-host disease following stem-cell transplantation.
Kurashima Y, Amiya T, Nochi T, Fujisawa K, Haraguchi T, Iba H, Tsutsui H, Sato S, Nakajima S, Iijima H, Kubo M, Kunisawa J, Kiyono H
Nature communications 2012;3:1034
Nature communications 2012;3:1034
CCR1/CCL5 (RANTES) receptor-ligand interactions modulate allogeneic T-cell responses and graft-versus-host disease following stem-cell transplantation.
Choi SW, Hildebrandt GC, Olkiewicz KM, Hanauer DA, Chaudhary MN, Silva IA, Rogers CE, Deurloo DT, Fisher JM, Liu C, Adams D, Chensue SW, Cooke KR
Blood 2007 Nov 1;110(9):3447-55
Blood 2007 Nov 1;110(9):3447-55
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of 10 ug of total cell lysate from A-375 cells with CCR1 polyclonal antibody (Cat # PAB0176) at 2 ug/mL dilution.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemistry (Formalin/PFA-Fixed Paraffin-Embedded Sections) staining of human spleen with CCR1 polyclonal antibody (Cat # PAB0176).
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Left image : Surface staining of mouse primary epidermal cells (B-6 mice, 30 wks) with CL 11-PE, CD11c PE-Cy5, and CCR1 polyclonal antibody (Cat # PAB0176) with CY5 goat anti rabbit secondary. Right image : CD11c+ CL II+ cells (Langerans), stained with rabbit isotype control (shaded histogram) were analyzed for CCR1 at 5 uL (blue histogram) and 10 uL (Green histogram). Data kindly provided by Corey Parlet, University of Iowa.
- Validation comment
- Flow Cytometry