PA5-22042

antibody from Invitrogen Antibodies

Targeting: DECR1 DECR, SDR18C1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22042

Provider

Invitrogen Antibodies

Product name

DECR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse liver, Rat heart.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of DECR1 was performed by separating 50 µg of rat tissue extract by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a DECR1 Polyclonal Antibody (Product # PA5-22042) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DECR1 Polyclonal Antibody (Product # PA5-22042). Sample (30 µg of whole cell lysate). Lane A: HepG2. 10% SDS PAGE. DECR1 Polyclonal Antibody (Product # PA5-22042) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using DECR1 Polyclonal Antibody (Product # PA5-22042). Sample (20 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. DECR1 Polyclonal Antibody (Product # PA5-22042) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DECR1 Polyclonal Antibody detects DECR1 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DECR1 protein stained by DECR1 Polyclonal Antibody (Product # PA5-22042) diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded NCI-N87 xenograft, using DECR1 (Product # PA5-22042) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.