H00003433-D01

antibody from Abnova Corporation

Targeting: IFIT2 cig42, G10P2, GARG-39, IFI-54, IFI54, ISG-54K

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003433-D01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003433-D01, RRID:AB_10661192

Product name

IFIT2 MaxPab rabbit polyclonal antibody (D01)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human IFIT2 protein.

Antigen sequence

MSENNKNSLESSLRQLKCHFTWNLMEGENSLDDFE
DKVFYRTEFQNREFKATMCNLLAYLKHLKGQNEAA
LECLRKAEELIQQEHADQAEIRSLVTWGNYAWVYY
HMGRLSDVQIYVDKVRHVCEKFSSPYRIESPELDC
EEGWTRLKCGGNQNERAKVCFEKALEKKPKNPEFT
SGLAIASYRLDNWPPSQNAIDPLRQAIRLNPDNQY
LKVLLALKLHKMREEGEEEGEGEKLVEEALEKAPG
VTDVLRSAAKFYRRKDEPDKAIELLKKALEYIPNN
AYLHCQIGCCYRAKVFQVMNLRENGMYGKRKLLEL
IGHAVAHLKKADEANDNLFRVCSILASLHALADQY
EEAEYYFQKEFSKELTPVAKQLLHLRYGNFQLYQM
KCEDKAIHHFIEGVKINQKSREKEKMKDKLQKIAK
MRLSKNGADSEALHVLAFLQELNEKMQQADEDSER
GLESGSLIPSASSWNGEWRIEMWCPLGYC


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IFIT2 expression in transfected 293T cell line (H00003433-T02) by IFIT2 MaxPab polyclonal antibody.Lane 1: IFIT2 transfected lysate(56.20 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to IFIT2 on HeLa cell. [antibody concentration 20 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Immunoprecipitation

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoprecipitation of IFIT2 transfected lysate using anti-IFIT2 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with IFIT2 MaxPab mouse polyclonal antibody (B01) (H00003433-B01).

Validation comment

Immunoprecipitation

Protocol

Protocol