12604-1-AP
antibody from Invitrogen Antibodies
Targeting: IFIT2
cig42, G10P2, GARG-39, IFI-54, IFI54, ISG-54K
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- 12604-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IFIT2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ATMCNLLAY LKHLKGQNEA ALECLRKAEE LIQQEHADQA EIRSLVTWGN YAWVYYHMGR LSDVQIYVDK VRHVCEKFSS PYRIESPELD CEEGWTRLKC GGNQNERAKV CFEKALEKKP KNPEFTSGLA IASYRLDNWP PSQNAIDPLR QAIRLNPDNQ YLKVLLALKL HKMREEGEEE GEGEKLVEEA LEKAPGVTDV LRSAAKFYRR KDEPDKAIEL LKKALEYIPN NAYLHCQIGC CYRAKVFQVM NLRENGMYGK RKLLELIGHA VAHLKKADEA NDNLFRVCSI LASLHALADQ YEEAEYYFQK EFSKELTPVA KQLLHLRYGN FQLYQMKCED KAIHHFIEGV (51-399 aa encoded by BC032839)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.27 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- A431 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP ( IFIT2 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HepG2 cells using 12604-1-AP (IFIT2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 12604-1-AP ( IFIT2 Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:300) with mouse skin tissue lysate 3200ug.