Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX629000 - Provider product page
- Provider
- GeneTex
- Product name
- ADAR1 antibody [GT1066]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ADAR1 antibody [GT1066] detects ADAR protein by Western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g A431 whole cell lysate/extractC. 30 ?g HeLa whole cell lysate/extractD. 30 ?g HepG2 whole cell lysate/extractE. 30 ?g A375 whole cell lysate/extract5 % SDS-PAGEADAR1 antibody [GT1066] (GTX629000) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with ADAR1 antibody [GT1066] (GTX629000) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ADAR1 antibody [GT1066] detects ADAR1 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde for 10 min.Green: ADAR1 protein stained by ADAR1 antibody [GT1066] (GTX629000) diluted at 1:100.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of ADAR1 protein from 293T whole cell extracts using 5 £gg of ADAR1 antibody [GT1066] (GTX629000).Western blot analysis was performed using ADAR1 antibody [GT1066] (GTX629000).EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.
