60074-1-Ig

antibody from Proteintech Group

Targeting: IFITM1 9-27, CD225, DSPA2a, IFI17

Provider product page for 60074-1-Ig

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Antibody data

Antibody Data
Antigen structure
References [71]
Comments [0]
Validations
Western blot [1]
Immunohistochemistry [1]

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Product number: 60074-1-Ig - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#60074-1-Ig, RRID:AB_2233405
Product name: IFITM1-Specific antibody
Antibody type: Monoclonal
Description: KD/KO validated IFITM1-Specific antibody (Cat. #60074-1-Ig) is a mouse monoclonal antibody that shows reactivity with human and has been validated for the following applications: IHC, IP, WB, ELISA.
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Isotype: IgG
Antibody clone number: 5B5E2
Vial size: 20ul, 150ul

IFITM1 protein structure - 60074-1-Ig shown in red.

Submitted references A novel type-2 innate lymphoid cell-based immunotherapy for cancer.

Saranchova I, Xia CW, Besoiu S, Finkel PL, Ellis SLS, Kari S, Munro L, Pfeifer CG, Fazli L, Gleave ME, Jefferies WA
Frontiers in immunology 2024;15:1317522

Mechanisms of mesothelial cell response to viral infections: HDAC1-3 inhibition blocks poly(I:C)-induced type I interferon response and modulates the mesenchymal/inflammatory phenotype.

Trionfetti F, Montaldo C, Caiello I, Bontempi G, Terri M, Tiberi M, Marchant V, Domenici A, Menè P, Cordani M, Zwergel C, Prencipe G, Ruiz-Ortega M, Valente S, Mai A, Tripodi M, Strippoli R
Frontiers in cellular and infection microbiology 2024;14:1308362

Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells.

Yang Y, Ding T, Cong Y, Luo X, Liu C, Gong T, Zhao M, Zheng X, Li C, Zhang Y, Zhou J, Ni C, Zhang X, Ji Z, Wu T, Yang S, Zhou Q, Wu D, Gong X, Zheng Q, Li X
Nature microbiology 2024 May;9(5):1256-1270

A diversity of novel type-2 innate lymphoid cell subpopulations revealed during tumour expansion.

Xia CW, Saranchova I, Finkel PL, Besoiu S, Munro L, Pfeifer CG, Haegert A, Lin YY, Le Bihan S, Collins C, Jefferies WA
Communications biology 2024 Jan 3;7(1):12

CRISPR activation as a platform to identify interferon stimulated genes with anti-viral function.

Kirby EN, Montin XB, Allen TP, Densumite J, Trowbridge BN, Beard MR
Innate immunity 2024 Feb;30(2-4):40-54

Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity.

Zhang YG, Zhang HX, Chen HW, Lv P, Su J, Chen YR, Fu ZF, Cui M
Journal of neuroinflammation 2023 Sep 27;20(1):216

MicroRNA-363-3p inhibits colorectal cancer progression by targeting interferon-induced transmembrane protein 1.

Wang Y, Bai SK, Zhang T, Liao CG
World journal of gastrointestinal oncology 2023 Sep 15;15(9):1556-1566

Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1.

Xu R, Lee YJ, Kim CH, Min GH, Kim YB, Park JW, Kim DH, Kim JH, Yim H
Journal of experimental & clinical cancer research : CR 2023 Nov 16;42(1):302

Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon.

Lu AY, Gustin A, Newhouse D, Gale M Jr
Journal of virology 2023 May 31;97(5):e0198222

Interferon-inducible phospholipids govern IFITM3-dependent endosomal antiviral immunity.

Unali G, Crivicich G, Pagani I, Abou-Alezz M, Folchini F, Valeri E, Matafora V, Reisz JA, Giordano AMS, Cuccovillo I, Butta GM, Donnici L, D'Alessandro A, De Francesco R, Manganaro L, Cittaro D, Merelli I, Petrillo C, Bachi A, Vicenzi E, Kajaste-Rudnitski A
The EMBO journal 2023 May 15;42(10):e112234

IFITM2 Presents Antiviral Response through Enhancing Type I IFN Signaling Pathway.

Chen L, Li X, Deng Y, Bi Y, Yan Z, Yang Y, Zhang X, Li H, Xie J, Feng R
Viruses 2023 Mar 28;15(4)

Caveats of Using Overexpression Approaches to Screen Cellular Host IFITM Proteins for Antiviral Activity.

Meischel T, Fritzlar S, Villalón-Letelier F, Smith JM, Brooks AG, Reading PC, Londrigan SL
Pathogens (Basel, Switzerland) 2023 Mar 27;12(4)

Cooperative sensing of mitochondrial DNA by ZBP1 and cGAS promotes cardiotoxicity.

Lei Y, VanPortfliet JJ, Chen YF, Bryant JD, Li Y, Fails D, Torres-Odio S, Ragan KB, Deng J, Mohan A, Wang B, Brahms ON, Yates SD, Spencer M, Tong CW, Bosenberg MW, West LC, Shadel GS, Shutt TE, Upton JW, Li P, West AP
Cell 2023 Jul 6;186(14):3013-3032.e22

IFITM1 inhibits trophoblast invasion and is induced in placentas associated with IFN-mediated pregnancy diseases.

Degrelle SA, Buchrieser J, Dupressoir A, Porrot F, Loeuillet L, Schwartz O, Fournier T
iScience 2023 Jul 21;26(7):107147

Dynamic states of cervical epithelia during pregnancy and epithelial barrier disruption.

Cooley A, Madhukaran S, Stroebele E, Colon Caraballo M, Wang L, Akgul Y, Hon GC, Mahendroo M
iScience 2023 Feb 17;26(2):105953

HIV-1 Nef Changes the Proteome of T Cells Extracellular Vesicles Depleting IFITMs and Other Antiviral Factors.

da Silva-Januário ME, da Costa CS, Tavares LA, Oliveira AK, Januário YC, de Carvalho AN, Cassiano MHA, Rodrigues RL, Miller ME, Palameta S, Arns CW, Arruda E, Paes Leme AF, daSilva LLP
Molecular & cellular proteomics : MCP 2023 Dec;22(12):100676

Prolonged Primary Rhinovirus Infection of Human Nasal Epithelial Cells Diminishes the Viral Load of Secondary Influenza H3N2 Infection via the Antiviral State Mediated by RIG-I and Interferon-Stimulated Genes.

Ong HH, Liu J, Oo Y, Thong M, Wang Y, Chow VT
Cells 2023 Apr 13;12(8)

IFITM3 blocks influenza virus entry by sorting lipids and stabilizing hemifusion.

Klein S, Golani G, Lolicato F, Lahr C, Beyer D, Herrmann A, Wachsmuth-Melm M, Reddmann N, Brecht R, Hosseinzadeh M, Kolovou A, Makroczyova J, Peterl S, Schorb M, Schwab Y, Brügger B, Nickel W, Schwarz US, Chlanda P
Cell host & microbe 2023 Apr 12;31(4):616-633.e20

Inhibition of Arenavirus Entry and Replication by the Cell-Intrinsic Restriction Factor ZMPSTE24 Is Enhanced by IFITM Antiviral Activity.

Stott-Marshall RJ, Foster TL
Frontiers in microbiology 2022;13:840885

Inhibitory and Stimulatory Effects of IL-32 on HIV-1 Infection.

Nasser H, Takahashi N, Eltalkhawy YM, Reda O, Lotfi S, Nasu K, Sakuragi JI, Suzu S
Journal of immunology (Baltimore, Md. : 1950) 2022 Sep 1;209(5):970-978

Intrinsic antiviral immunity of barrier cells revealed by an iPSC-derived blood-brain barrier cellular model.

Cheng Y, Medina A, Yao Z, Basu M, Natekar JP, Lang J, Sanchez E, Nkembo MB, Xu C, Qian X, Nguyen PTT, Wen Z, Song H, Ming GL, Kumar M, Brinton MA, Li MMH, Tang H
Cell reports 2022 May 31;39(9):110885

Eukaryotic Initiation Factor 5A2 Regulates Expression of Antiviral Genes.

Farache D, Liu L, Lee ASY
Journal of molecular biology 2022 May 30;434(10):167564

FTO Suppresses STAT3 Activation and Modulates Proinflammatory Interferon-Stimulated Gene Expression.

McFadden MJ, Sacco MT, Murphy KA, Park M, Gokhale NS, Somfleth KY, Horner SM
Journal of molecular biology 2022 Mar 30;434(6):167247

A novel domain within the CIL regulates egress of IFITM3 from the Golgi and reveals a regulatory role of IFITM3 on the secretory pathway.

Zhong L, Song Y, Marziali F, Uzbekov R, Nguyen XN, Journo C, Roingeard P, Cimarelli A
Life science alliance 2022 Jul;5(7)

Innate immune regulation in HIV latency models.

Olson RM, Gornalusse G, Whitmore LS, Newhouse D, Tisoncik-Go J, Smith E, Ochsenbauer C, Hladik F, Gale M Jr
Retrovirology 2022 Jul 8;19(1):15

The Role of IFITM Proteins in Tick-Borne Encephalitis Virus Infection.

Chmielewska AM, Gómez-Herranz M, Gach P, Nekulova M, Bagnucka MA, Lipińska AD, Rychłowski M, Hoffmann W, Król E, Vojtesek B, Sloan RD, Bieńkowska-Szewczyk K, Hupp T, Ball K
Journal of virology 2022 Jan 12;96(1):e0113021

Assessing Mitochondrial DNA Release into the Cytosol and Subsequent Activation of Innate Immune-related Pathways in Mammalian Cells.

Bryant JD, Lei Y, VanPortfliet JJ, Winters AD, West AP
Current protocols 2022 Feb;2(2):e372

Rapalogs downmodulate intrinsic immunity and promote cell entry of SARS-CoV-2.

Shi G, Chiramel AI, Li T, Lai KK, Kenney AD, Zani A, Eddy A, Majdoul S, Zhang L, Dempsey T, Beare PA, Kar S, Yewdell JW, Best SM, Yount JS, Compton AA
bioRxiv : the preprint server for biology 2022 Aug 30;

RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers.

Abraham HG, Ulintz PJ, Goo L, Yates JA, Little AC, Bao L, Wu Z, Merajver SD
Frontiers in oncology 2021;11:712041

IFITM Proteins That Restrict the Early Stages of Respiratory Virus Infection Do Not Influence Late-Stage Replication.

Meischel T, Fritzlar S, Villalon-Letelier F, Tessema MB, Brooks AG, Reading PC, Londrigan SL
Journal of virology 2021 Sep 27;95(20):e0083721

Long Noncoding RNA IFITM4P Regulates Host Antiviral Responses by Acting as a Competing Endogenous RNA.

Xiao M, Chen Y, Wang S, Liu S, Rai KR, Chen B, Li F, Li Y, Maarouf M, Chen JL
Journal of virology 2021 Oct 13;95(21):e0027721

Post-transcriptional regulation of antiviral gene expression by N6-methyladenosine.

McFadden MJ, McIntyre ABR, Mourelatos H, Abell NS, Gokhale NS, Ipas H, Xhemalçe B, Mason CE, Horner SM
Cell reports 2021 Mar 2;34(9):108798

IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.

Xu X, Wu Y, Yi K, Hu Y, Ding W, Xing C
International journal of molecular medicine 2021 Jun;47(6)

Human IFITM3 restricts chikungunya virus and Mayaro virus infection and is susceptible to virus-mediated counteraction.

Franz S, Pott F, Zillinger T, Schüler C, Dapa S, Fischer C, Passos V, Stenzel S, Chen F, Döhner K, Hartmann G, Sodeik B, Pessler F, Simmons G, Drexler JF, Goffinet C
Life science alliance 2021 Jul;4(7)

ETV7 limits antiviral gene expression and control of influenza viruses.

Froggatt HM, Harding AT, Chaparian RR, Heaton NS
Science signaling 2021 Jul 13;14(691)

Loss of Mitochondrial Protease CLPP Activates Type I IFN Responses through the Mitochondrial DNA-cGAS-STING Signaling Axis.

Torres-Odio S, Lei Y, Gispert S, Maletzko A, Key J, Menissy SS, Wittig I, Auburger G, West AP
Journal of immunology (Baltimore, Md. : 1950) 2021 Apr 15;206(8):1890-1900

The Polybasic Cleavage Site in SARS-CoV-2 Spike Modulates Viral Sensitivity to Type I Interferon and IFITM2.

Winstone H, Lista MJ, Reid AC, Bouton C, Pickering S, Galao RP, Kerridge C, Doores KJ, Swanson CM, Neil SJD
Journal of virology 2021 Apr 12;95(9)

p53 promotes ZDHHC1-mediated IFITM3 palmitoylation to inhibit Japanese encephalitis virus replication.

Wang X, Wu Z, Li Y, Yang Y, Xiao C, Liu X, Xiang X, Wei J, Shao D, Liu K, Deng X, Wu J, Qiu Y, Li B, Ma Z
PLoS pathogens 2020 Oct;16(10):e1009035

The mRNA Cap 2'-O-Methyltransferase CMTR1 Regulates the Expression of Certain Interferon-Stimulated Genes.

Williams GD, Gokhale NS, Snider DL, Horner SM
mSphere 2020 May 13;5(3)

IFITM3 Reduces Retroviral Envelope Abundance and Function and Is Counteracted by glycoGag.

Ahi YS, Yimer D, Shi G, Majdoul S, Rahman K, Rein A, Compton AA
mBio 2020 Jan 21;11(1)

Syncytia formation by SARS-CoV-2-infected cells.

Buchrieser J, Dufloo J, Hubert M, Monel B, Planas D, Rajah MM, Planchais C, Porrot F, Guivel-Benhassine F, Van der Werf S, Casartelli N, Mouquet H, Bruel T, Schwartz O
The EMBO journal 2020 Dec 1;39(23):e106267

LY6E Restricts Entry of Human Coronaviruses, Including Currently Pandemic SARS-CoV-2.

Zhao X, Zheng S, Chen D, Zheng M, Li X, Li G, Lin H, Chang J, Zeng H, Guo JT
Journal of virology 2020 Aug 31;94(18)

IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise.

Buchrieser J, Degrelle SA, Couderc T, Nevers Q, Disson O, Manet C, Donahue DA, Porrot F, Hillion KH, Perthame E, Arroyo MV, Souquere S, Ruigrok K, Dupressoir A, Heidmann T, Montagutelli X, Fournier T, Lecuit M, Schwartz O
Science (New York, N.Y.) 2019 Jul 12;365(6449):176-180

Identifying key barriers in cationic polymer gene delivery to human T cells.

Olden BR, Cheng E, Cheng Y, Pun SH
Biomaterials science 2019 Feb 26;7(3):789-797

Analysis of IFITM-IFITM Interactions by a Flow Cytometry-Based FRET Assay.

Winkler M, Wrensch F, Bosch P, Knoth M, Schindler M, Gärtner S, Pöhlmann S
International journal of molecular sciences 2019 Aug 8;20(16)

Influenza A Virus Facilitates Its Infectivity by Activating p53 to Inhibit the Expression of Interferon-Induced Transmembrane Proteins.

Wang B, Lam TH, Soh MK, Ye Z, Chen J, Ren EC
Frontiers in immunology 2018;9:1193

IFITM proteins inhibit HIV-1 protein synthesis.

Lee WJ, Fu RM, Liang C, Sloan RD
Scientific reports 2018 Sep 28;8(1):14551

mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3.

Shi G, Ozog S, Torbett BE, Compton AA
Proceedings of the National Academy of Sciences of the United States of America 2018 Oct 23;115(43):E10069-E10078

A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery.

Roesch F, OhAinle M, Emerman M
Retrovirology 2018 Mar 20;15(1):26

Multi-modal Potentiation of Oncolytic Virotherapy by Vanadium Compounds.

Selman M, Rousso C, Bergeron A, Son HH, Krishnan R, El-Sayes NA, Varette O, Chen A, Le Boeuf F, Tzelepis F, Bell JC, Crans DC, Diallo JS
Molecular therapy : the journal of the American Society of Gene Therapy 2018 Jan 3;26(1):56-69

Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition.

Selman M, Ou P, Rousso C, Bergeron A, Krishnan R, Pikor L, Chen A, Keller BA, Ilkow C, Bell JC, Diallo JS
Science translational medicine 2018 Jan 24;10(425)

Cyclosporine H Overcomes Innate Immune Restrictions to Improve Lentiviral Transduction and Gene Editing In Human Hematopoietic Stem Cells.

Petrillo C, Thorne LG, Unali G, Schiroli G, Giordano AMS, Piras F, Cuccovillo I, Petit SJ, Ahsan F, Noursadeghi M, Clare S, Genovese P, Gentner B, Naldini L, Towers GJ, Kajaste-Rudnitski A
Cell stem cell 2018 Dec 6;23(6):820-832.e9

Rapid reversal of innate immune dysregulation in blood of patients and livers of humanized mice with HCV following DAA therapy.

Burchill MA, Roby JA, Crochet N, Wind-Rotolo M, Stone AE, Edwards MG, Dran RJ, Kriss MS, Gale M Jr, Rosen HR
PloS one 2017;12(10):e0186213

Zika virus induces massive cytoplasmic vacuolization and paraptosis-like death in infected cells.

Monel B, Compton AA, Bruel T, Amraoui S, Burlaud-Gaillard J, Roy N, Guivel-Benhassine F, Porrot F, Génin P, Meertens L, Sinigaglia L, Jouvenet N, Weil R, Casartelli N, Demangel C, Simon-Lorière E, Moris A, Roingeard P, Amara A, Schwartz O
The EMBO journal 2017 Jun 14;36(12):1653-1668

IFITM1 targets HIV-1 latently infected cells for antibody-dependent cytolysis.

Raposo RAS, de Mulder Rougvie M, Paquin-Proulx D, Brailey PM, Cabido VD, Zdinak PM, Thomas AS, Huang SH, Beckerle GA, Jones RB, Nixon DF
JCI insight 2017 Jan 12;2(1):e85811

Interferon-Induced Transmembrane Protein 3 Inhibits Hantaan Virus Infection, and Its Single Nucleotide Polymorphism rs12252 Influences the Severity of Hemorrhagic Fever with Renal Syndrome.

Xu-Yang Z, Pei-Yu B, Chuan-Tao Y, Wei Y, Hong-Wei M, Kang T, Chun-Mei Z, Ying-Feng L, Xin W, Ping-Zhong W, Chang-Xing H, Xue-Fan B, Ying Z, Zhan-Sheng J
Frontiers in immunology 2016;7:535

Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.

Foster TL, Wilson H, Iyer SS, Coss K, Doores K, Smith S, Kellam P, Finzi A, Borrow P, Hahn BH, Neil SJD
Cell host & microbe 2016 Oct 12;20(4):429-442

The IFITMs Inhibit Zika Virus Replication.

Savidis G, Perreira JM, Portmann JM, Meraner P, Guo Z, Green S, Brass AL
Cell reports 2016 Jun 14;15(11):2323-30

Global analysis of DNA methylation in hepatocellular carcinoma by a liquid hybridization capture-based bisulfite sequencing approach.

Gao F, Liang H, Lu H, Wang J, Xia M, Yuan Z, Yao Y, Wang T, Tan X, Laurence A, Xu H, Yu J, Xiao W, Chen W, Zhou M, Zhang X, Chen Q, Chen X
Clinical epigenetics 2015;7(1):86

Human cytomegalovirus exploits interferon-induced transmembrane proteins to facilitate morphogenesis of the virion assembly compartment.

Xie M, Xuan B, Shan J, Pan D, Sun Y, Shan Z, Zhang J, Yu D, Li B, Qian Z
Journal of virology 2015 Mar;89(6):3049-61

Human respiratory syncytial virus infection is inhibited by IFN-induced transmembrane proteins.

Zhang W, Zhang L, Zan Y, Du N, Yang Y, Tien P
The Journal of general virology 2015 Jan;96(Pt 1):170-182

IFITM3-containing exosome as a novel mediator for anti-viral response in dengue virus infection.

Zhu X, He Z, Yuan J, Wen W, Huang X, Hu Y, Lin C, Pan J, Li R, Deng H, Liao S, Zhou R, Wu J, Li J, Li M
Cellular microbiology 2015 Jan;17(1):105-18

IFITM proteins inhibit entry driven by the MERS-coronavirus spike protein: evidence for cholesterol-independent mechanisms.

Wrensch F, Winkler M, Pöhlmann S
Viruses 2014 Sep 26;6(9):3683-98

IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.

Tartour K, Appourchaux R, Gaillard J, Nguyen XN, Durand S, Turpin J, Beaumont E, Roch E, Berger G, Mahieux R, Brand D, Roingeard P, Cimarelli A
Retrovirology 2014 Nov 25;11:103

Interferon induction of IFITM proteins promotes infection by human coronavirus OC43.

Zhao X, Guo F, Liu F, Cuconati A, Chang J, Block TM, Guo JT
Proceedings of the National Academy of Sciences of the United States of America 2014 May 6;111(18):6756-61

IFITM proteins incorporated into HIV-1 virions impair viral fusion and spread.

Compton AA, Bruel T, Porrot F, Mallet A, Sachse M, Euvrard M, Liang C, Casartelli N, Schwartz O
Cell host & microbe 2014 Dec 10;16(6):736-47

Amphotericin B increases influenza A virus infection by preventing IFITM3-mediated restriction.

Lin TY, Chin CR, Everitt AR, Clare S, Perreira JM, Savidis G, Aker AM, John SP, Sarlah D, Carreira EM, Elledge SJ, Kellam P, Brass AL
Cell reports 2013 Nov 27;5(4):895-908

The CD225 domain of IFITM3 is required for both IFITM protein association and inhibition of influenza A virus and dengue virus replication.

John SP, Chin CR, Perreira JM, Feeley EM, Aker AM, Savidis G, Smith SE, Elia AE, Everitt AR, Vora M, Pertel T, Elledge SJ, Kellam P, Brass AL
Journal of virology 2013 Jul;87(14):7837-52

IFITM1 is a tight junction protein that inhibits hepatitis C virus entry.

Wilkins C, Woodward J, Lau DT, Barnes A, Joyce M, McFarlane N, McKeating JA, Tyrrell DL, Gale M Jr
Hepatology (Baltimore, Md.) 2013 Feb;57(2):461-9

Innate immune tolerance and the role of kupffer cells in differential responses to interferon therapy among patients with HCV genotype 1 infection.

Lau DT, Negash A, Chen J, Crochet N, Sinha M, Zhang Y, Guedj J, Holder S, Saito T, Lemon SM, Luxon BA, Perelson AS, Gale M Jr
Gastroenterology 2013 Feb;144(2):402-413.e12

The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus.

Brass AL, Huang IC, Benita Y, John SP, Krishnan MN, Feeley EM, Ryan BJ, Weyer JL, van der Weyden L, Fikrig E, Adams DJ, Xavier RJ, Farzan M, Elledge SJ
Cell 2009 Dec 24;139(7):1243-54

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: K-562 cells were subjected to SDS PAGE followed by western blot with 60074-1-Ig(IFITM1 antibody) at dilution of 1:1000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: The IFITM1-Specific antibody from Proteintech is a mouse monoclonal antibody to a recombinant protein of human IFITM1-Specific. This antibody recognizes human antigen. The IFITM1-Specific antibody has been validated for the following applications: ELISA, WB, IHC analysis.
Appendix: 60074-1-Ig.pdf

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