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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00005080-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005080-D01, RRID:AB_10717226
- Product name
- PAX6 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human PAX6 protein.
- Antigen sequence
MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHS
GARPCDISRILQVSNGCVSKILGRYYETGSIRPRA
IGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDR
LLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGAD
GMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQD
GCQQQEGGGENTNSISSNGEDSDEAQMRLQLKRKL
QRNRTSFTQEQIEALEKEFERTHYPDVFARERLAA
KIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASN
TPSHIPISSSFSTSVYQPIPQPTTPVSSFTSGSML
GRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQ
TSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQP
MGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPR
LQ- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- PAX6 MaxPab rabbit polyclonal antibody. Western Blot analysis of PAX6 expression in mouse spleen.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of PAX6 expression in transfected 293T cell line (H00005080-T02) by PAX6 MaxPab polyclonal antibody.Lane 1: PAX6 transfected lysate(46.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of PAX6 transfected lysate using anti-PAX6 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with PAX6 MaxPab mouse polyclonal antibody (B01) (H00005080-B01).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
