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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC8150P-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Pax6 PE-conjugated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human Pax6 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human (rh) Pax1, rhPax2, rhPax3, rhPax4, rhPax5, and rhPax7 is observed.
- Reactivity
- Human
- Host
- Sheep
- Antigen sequence
P26367- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Pax6 in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Sheep Anti-Human Pax6 PE-conjugated Affinity-Purified Polyclonal Antibody (Catalog # IC8150P, filled histogram) or Sheep IgG PE-conjugated control Antibody (Catalog # IC016P, open histogram). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.
