Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 655701 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#655701, RRID:AB_2562201
- Product name
- Purified anti-IRF3
- Antibody type
- Monoclonal
- Antigen
- Partial IRF-3 recombinant protein (200-427 a.a.)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 12A4A35
- Vial size
- 25 µg
- Concentration
- 0.5 mg/ml
- Storage
- 4° C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- U937 cell (lane 1) and mouse spleen cell (lane 2) extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-IRF3 (clone 12A4A35) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated