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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54481 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- IRF3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G affinity
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.05 µl, 0.2 µl
- Storage
- Aliquot the IRF3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) monkey COS-7 and human 2) Daudi, 3) HepG2, 4) HT-29, 5) Jurkat and 6) MCF7 cell lysate with IRF3 antibody. Predicted molecular weight ~47 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of fixed and permeabilized human HeLa cells with IRF3 antibody (green), DAPI nuclear stain (blue) and anti-Actin (red).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human kidney tissue with IRF3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human Jurkat cells with IRF3 antibody; Blue=isotype control, Green= IRF3 antibody.
