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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA5-54483 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VPS13A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KCGLVMLNNL VKAFTEAATG SSADFVKDLA PFMILNSLGL TISVSPSDSF SVLNIPMAKS YVLKNGESLS MDYIRTKDND HFNAMTSLSS KLFFILLTPV NHSTADKIPL TKVGRRLYTV RHRESGVERS IVCQIDTVEG SKKVTIRSPV
- Concentration
- 0.4 mg/mL
Submitted references Unraveling the Spatiotemporal Distribution of VPS13A in the Mouse Brain.
García-García E, Chaparro-Cabanillas N, Coll-Manzano A, Carreras-Caballé M, Giralt A, Del Toro D, Alberch J, Masana M, Rodríguez MJ
International journal of molecular sciences 2021 Dec 1;22(23)
International journal of molecular sciences 2021 Dec 1;22(23)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of VPS13A in human cell line U-2 OS shows positivity in mitochondria. Samples were probed using a VPS13A Polyclonal Antibody (Product # PA5-54483).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of VPS13A in human duodenum using a VPS13A Polyclonal Antibody (Product # PA5-54483) shows strong granular cytoplasmic positivity in glandular cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 VPS13A is mainly present in the soma of the neurons, colocalizes with endoplasmic reticulum and mitochondria, and is found in synaptosomal fraction lysates. ( A ) Representative confocal images of VPS13A immunostaining in cortical primary neurons. ( B ) Orthogonal view from different planes of representative confocal images of VPS13A/Calnexin colocalization in cortical primary neurons. ( C ) Colocalization scatter plot showing the relationships between the signals in each pixel for VPS13A and Calnexin. ( D ) Orthogonal view from different planes of representative confocal images of VPS13A/TOMM20 colocalization in cortical primary neurons. ( E ) Colocalization scatter plot showing the relationships between the signals in each pixel for VPS13A and TOMM20. Scale bar, ( A , B , D ), 10 um; detail in ( A ), 5 um. ( F ) Orthogonal view from different planes of representative confocal images of VPS13A/F-Actin localization in cortical primary neurons. Scale bar 5 um. ( G ) Homogenate (H) and synaptosomal fractions (S) of mouse cortex were analyzed by Western blot. SV2A (pre-synaptic protein) and PSD-95 (post-synaptic protein) were used as the control for synaptosomal fraction isolation. Actin was used as a loading control. Data representing mean +- SEM and differences were analyzed by Student's t -test; *** p < 0.001. n = 8.
