AF1032

antibody from R&D Systems

Targeting: CDH17 cadherin, HPT-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AF1032

Provider

R&D Systems

Product name

Human Cadherin-17 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Cadherin-17 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Cadherin-8, rhE-Cadherin, rhP-Cadherin, and rhVE-Cadherin is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

CAA58231


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human and Mouse Cadherin-17 by Western Blot. Western blot shows lysates of human colon tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1032) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cadherin-17 at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cadherin-17 in Human Colon. Cadherin-17 was detected in immersion fixed paraffin-embedded sections of human colon array using Goat Anti-Human Cadherin-17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1032) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.