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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- AF3756a - Provider product page
- Provider
- Abgent
- Product name
- GEM (aa34-46) Antibody (internal region)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide-affinity purified goat antibody
- Reactivity
- Mouse, Rat, Bovine, Canine, Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1mg
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -1362°C in small aliquots to prevent freeze-thaw cycles.
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Supportive validation
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- "HEK293 lysate overexpressing full-length Human GEM (HA tagged), mock-transfected HEK293 (EV) and HEK293 transiently expressing GEM-related genes (Rem, Rem2L and Rad) probed with AF3756a (1ug/ml). The same lysates probed with anti-HA tag antibody in the lower panel."
- Submitted by
- Abgent (provider)
- Main image
- Experimental details
- AF3756a (1 µg/ml) staining of Human Spleen lysate (35 µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
