LS-C313397

antibody from LSBio

Targeting: IL18 IGIF, IL-18, IL-1g, IL1F4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C313397

Provider

LSBio

Product name

IL18 Antibody (aa37-193) LS-C313397

Antibody type

Polyclonal

Description

Immunogen affinity purified

Reactivity

Human

Host

Rabbit

Isotype

IgG

Storage

At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

WB of IL18 antibody. All lanes: Anti-IL18 at 0.5ug/ml. WB: Recombinant Human IL18 Protein 0.5ng. Predicted bind size: 24KD. Observed bind size: 24KD.

Immunocytochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

ICC analysis of IL18 using anti-IL18 antibody. IL18 was detected in immunocytochemical section of blood cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL18 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

ICC analysis of IL18 using anti-IL18 antibody. IL18 was detected in immunocytochemical section of blood cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-IL18 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IL18 antibody. IHC(P): Human Tonsil Tissue.

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IL18 antibody. IHC(P): Human Tonsil Tissue.