Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [2]
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Validation data
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- Product number
- PA5-19131 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL-18 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Molecular Characteristics, Clinical Implication, and Cancer Immunity Interactions of Pyroptosis-Related Genes in Breast Cancer.
CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.
Xu D, Ji Z, Qiang L
Frontiers in medicine 2021;8:702638
Frontiers in medicine 2021;8:702638
CPTP: A sphingolipid transfer protein that regulates autophagy and inflammasome activation.
Mishra SK, Gao YG, Deng Y, Chalfant CE, Hinchcliffe EH, Brown RE
Autophagy 2018;14(5):862-879
Autophagy 2018;14(5):862-879
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot staining of HeLa cell lysate using Product # PA5-19131 at a concentration of 0.1 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 9 Validation of the expression of GSDMC, IL-18 , and TIRAP in normal breast cells (MCF-10A) and breast cancer cells (MDA-MB-231 and HCC70). (A-D) Western blot detecting the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. *** p < 0.001; **** p < 0.0001 . (E-G) Immunofluorescence of the expression of GSDMC, IL-18, and TIRAP in MCF-10A, MDA-MB-231, and HCC70 cells. Scale bar, 5 mum; magnification 200 x.