GTX81850

antibody from GeneTex

Targeting: JCHAIN IGCJ, IGJ, JCH

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX81850

Provider

GeneTex

Proper citation

GeneTex Cat#GTX81850, RRID:AB_11176486

Product name

Human J chain antibody, N-term

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Storage

Keep as concentrated solution. For short-term storage, store at 4¢X C (up to 10 days). For long-term storage, aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot analysis of IGJ antibody (N-term) (GTX81850) in mouse spleen lysates (35ug/lane). IGJ (arrow) was detected using the purified Pab.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded mouse spleen reacted with IGJ Antibody (N-term)(GTX81850), which was peroxidase-conjμgated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Flow cytometry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IGJ Antibody (N-term) (GTX81850) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjμgated goat-anti-rabbit secondary antibodies were used for the analysis.