13874-1-AP

antibody from Invitrogen Antibodies

Targeting: S1PR5 Edg-8, EDG8

Western blot (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

13874-1-AP

Provider

Invitrogen Antibodies

Product name

S1PR5/EDG8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: ARIYCQVRA NARRLPARPG TAGTTSTRAR RKPRSLALLR TLSVVLLAFV ACWGPLFLLL LLDVACPART CPVLLQADPF LGLAMANSLL NPIIYTLTNR DLRHALLRLV CCGRHSCGRD PSGSQQSASA AEASGGLRRC LPPGLDGSFS GSERSSPQRD GLDTSGSTGS PGAPTAARTL VSEPAAD (213-398 aa encoded by BC034703 )

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse brain tissue were subjected to SDS PAGE followed by western blot with 13874-1-AP ( S1PR5/EDG8 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HeLa cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Jurkat cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK-293 cells were subjected to SDS PAGE followed by western blot with 13874-1-AP (S1PR5/EDG8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

1X10^6 SH-SY5Y cells were stained with 0.2ug S1PR5/EDG8 antibody (13874-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.