Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [6]
- Flow cytometry [1]
- Other assay [1]
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Validation data
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- Product number
- 18099-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TRAF3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: KMDSPGALQ TNPPLKLHTD RSAGTPVFVP EQGGYKEKFV KTVEDKYKCE KCHLVLCSPK QTECGHRFCE SCMAALLSSS SPKCTACQES IVKDKVFKDN CCKREILALQ IYCRNESRGC AEQLMLGHLL VHLKNDCHFE ELPCVRPDCK EKVLRKDLRD HVEKACKYRE ATCSHCKSQV PMIALQKHED TDCPCVVVSC PHKCSVQTLL RSELSAHLSE CVNAPSTCSF KRYGCVFQGT NQQIKAHEAS SAVQHVNLLK EWSNSLEKKV SLLQNESVEK NKSIQSLHNQ ICSFEIEIER QKEMLRNNES KILHLQRVID SQAEKLKELD KEIRPFRQNW EEADSMK (6-351 aa encoded by BC075087)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.42 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB results of 18099-1-AP (anti TRAF3).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- WB result of TRAF3 antibody (18099-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRAF3 transfected HEK-293 cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HeLa cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP ( TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 18099-1-AP (TRAF3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- 1X10^6 Jurkat cells were stained with 0.2ug TRAF3 antibody (18099-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-TRAF3 (IP:18099-1-AP, 4ug; Detection:18099-1-AP 1:1000) with Raji cells lysate 3160 ug.