Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [8]
- Immunocytochemistry [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX103232 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103232, RRID:AB_1950516
- Product name
- GRP94 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Artocarpin induces cell apoptosis in human osteosarcoma cells through endoplasmic reticulum stress and reactive oxygen species.
Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes.
A Novel Luciferase Assay For Sensitively Monitoring Myocilin Variants in Cell Culture.
Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line.
Proteomic analysis of secreted proteins by human bronchial epithelial cells in response to cadmium toxicity.
Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.
Inhibition of reverse-mode sodium-calcium exchanger activity and apoptosis by levosimendan in human cardiomyocyte progenitor cell-derived cardiomyocytes after anoxia and reoxygenation.
Identification of minimum carbohydrate moiety in N-glycosylation sites of brain endothelial cell glycoprotein 96 for interaction with Escherichia coli K1 outer membrane protein A.
Angiotensin II receptor type 1--a novel target for preventing neonatal meningitis in mice by Escherichia coli K1.
Transcriptional activation of endoplasmic reticulum chaperone GRP78 by HCMV IE1-72 protein.
Lee CW, Chi MC, Chang TM, Liu JF
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Journal of cellular physiology 2019 Aug;234(8):13157-13168
Pro inflammatory stimuli enhance the immunosuppressive functions of adipose mesenchymal stem cells-derived exosomes.
Domenis R, Cifù A, Quaglia S, Pistis C, Moretti M, Vicario A, Parodi PC, Fabris M, Niazi KR, Soon-Shiong P, Curcio F
Scientific reports 2018 Sep 6;8(1):13325
Scientific reports 2018 Sep 6;8(1):13325
A Novel Luciferase Assay For Sensitively Monitoring Myocilin Variants in Cell Culture.
Zadoo S, Nguyen A, Zode G, Hulleman JD
Investigative ophthalmology & visual science 2016 Apr 1;57(4):1939-50
Investigative ophthalmology & visual science 2016 Apr 1;57(4):1939-50
Characterization of resistance to rhabdovirus and retrovirus infection in a human myeloid cell line.
Boso G, Somia NV
PloS one 2015;10(3):e0121455
PloS one 2015;10(3):e0121455
Proteomic analysis of secreted proteins by human bronchial epithelial cells in response to cadmium toxicity.
Chen DJ, Xu YM, Zheng W, Huang DY, Wong WY, Tai WC, Cho YY, Lau AT
Proteomics 2015 Sep;15(17):3075-86
Proteomics 2015 Sep;15(17):3075-86
Genetic ablation of N-linked glycosylation reveals two key folding pathways for R345W fibulin-3, a secreted protein associated with retinal degeneration.
Hulleman JD, Kelly JW
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Feb;29(2):565-75
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Feb;29(2):565-75
Inhibition of reverse-mode sodium-calcium exchanger activity and apoptosis by levosimendan in human cardiomyocyte progenitor cell-derived cardiomyocytes after anoxia and reoxygenation.
Li PC, Yang YC, Hwang GY, Kao LS, Lin CY
PloS one 2014;9(2):e85909
PloS one 2014;9(2):e85909
Identification of minimum carbohydrate moiety in N-glycosylation sites of brain endothelial cell glycoprotein 96 for interaction with Escherichia coli K1 outer membrane protein A.
Krishnan S, Prasadarao NV
Microbes and infection 2014 Jul;16(7):540-52
Microbes and infection 2014 Jul;16(7):540-52
Angiotensin II receptor type 1--a novel target for preventing neonatal meningitis in mice by Escherichia coli K1.
Krishnan S, Shanmuganathan MV, Behenna D, Stoltz BM, Prasadarao NV
The Journal of infectious diseases 2014 Feb 1;209(3):409-19
The Journal of infectious diseases 2014 Feb 1;209(3):409-19
Transcriptional activation of endoplasmic reticulum chaperone GRP78 by HCMV IE1-72 protein.
Shi-Chen Ou D, Lee SB, Chu CS, Chang LH, Chung BC, Juan LJ
Cell research 2011 Apr;21(4):642-53
Cell research 2011 Apr;21(4):642-53
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Supportive validation
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- Experimental details
- Sample (50 ?g of whole cell lysate) A: Mouse brain 7.5% SDS PAGE GTX103232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate)A: 293T 7.5% SDS PAGEGTX103232 diluted at 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX103232 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRP94 antibody detects GRP94 protein by western blot analysis. Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRP94 antibody detects GRP94 protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and GRP94 knockout (KO) 293T cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRP94 antibody (GTX103232) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- GRP94 antibody detects GRP94 protein by Western blot analysis. Various whole cell extracts (30 £gg) were separated by 7.5 % SDS-PAGE, and blotted with GRP94 antibody (GTX103232) diluted by 1:1000
Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using GRP94(GTX103232) antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2500.
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- Experimental details
- GRP94 antibody detects GRP94 protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: GRP94 protein stained by GRP94 antibody (GTX103232) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
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- Experimental details
- Immunoprecipitation of GRP94 protein from HeLa whole cell extracts using 5 £gg of GRP94 antibody (GTX103232).Western blot analysis was performed using GRP94 antibody (GTX103232).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded HEP3B xenograft, using GRP94(GTX103232) antibody at 1:100 dilution.
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- GeneTex (provider)
- Main image
- Experimental details
- GRP94 antibody detects GRP94 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. GRP94 antibody (GTX103232) diluted at 1:500.