AMAb91019

antibody from Atlas Antibodies

Targeting: HSP90B1 GP96, GRP94, TRA1

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AMAb91019

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#AMAb91019, RRID:AB_2665765

Product name

Anti-HSP90B1

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

SGNMERIMKAQAYQTGKDISTNYYASQKKTFEINP
RHPLIRDMLRRIKEDEDDKTVLDLAVVLFETATLR
SGYLLPDTKAYGDRIERMLRLSLNIDPDAKVEEEP
EEEPEETAEDTTEDTEQDEDEEMDVGTDEEEETAK
ESTA


Epitope

Binds to an epitope located within the peptide sequence EEEPEETAED as determined by overlapping synthetic peptides.

Isotype

IgG

Antibody clone number

CL2647

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10Lane 2: Human cell line U-251

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis in human cell line HeLa, human cell line HEK 293, human cell line A-431 and human cell line HepG2.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from U-251 cells, transfected with: control siRNA, target specific siRNA probe #1, target specific siRNA probe #2, using Anti-HSP90B1 monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-PPIB monoclonal antibody was used as loading control.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in HeLa cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in A431 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in MCF7 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in U2OS cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescence staining in U251 cell line with Anti-HSP90B1 monoclonal antibody, showing specific staining of endoplasmic reticulum in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Sample type

HUMAN

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human prostate shows strong cytoplasmic immunoreactivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cortex shows strong cytoplasmic positivity in neurons.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human rectum shows strong cytoplasmic immunoreactivity in glandular and lymphoid cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.