Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- V2908 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- GRP94 Antibody
- Antibody type
- Monoclonal
- Antigen
- Purified glucose regulated protein 94 from chicken oviducts was used as the immunogen for the GRP94 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian
- Host
- Rat
- Conjugate
- Unconjugated
- Antibody clone number
- 9G10.F8.2
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- Store the GRP94 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references HSP60 is transported through the secretory pathway of 3-MCA-induced fibrosarcoma tumour cells and undergoes N-glycosylation.
Absence of caveolin-1 alters heat shock protein expression in spontaneous mammary tumors driven by Her-2/neu expression.
Molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer.
Human plasmacytoid dendritic cells interact with gp96 via CD91 and regulate inflammatory responses.
Induction of heat shock protein gp96 by immune cytokines.
Peptide mimotopes of oncoproteins as therapeutic agents in breast cancer.
Hayoun D, Kapp T, Edri-Brami M, Ventura T, Cohen M, Avidan A, Lichtenstein RG
The FEBS journal 2012 Jun;279(12):2083-95
The FEBS journal 2012 Jun;279(12):2083-95
Absence of caveolin-1 alters heat shock protein expression in spontaneous mammary tumors driven by Her-2/neu expression.
Ciocca DR, Cuello-CarriĆ³n FD, Natoli AL, Restall C, Anderson RL
Histochemistry and cell biology 2012 Feb;137(2):187-94
Histochemistry and cell biology 2012 Feb;137(2):187-94
Molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer.
Chiu CC, Lin CY, Lee LY, Chen YJ, Lu YC, Wang HM, Liao CT, Chang JT, Cheng AJ
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jul 15;17(14):4629-41
Clinical cancer research : an official journal of the American Association for Cancer Research 2011 Jul 15;17(14):4629-41
Human plasmacytoid dendritic cells interact with gp96 via CD91 and regulate inflammatory responses.
De Filippo A, Binder RJ, Camisaschi C, Beretta V, Arienti F, Villa A, Della Mina P, Parmiani G, Rivoltini L, Castelli C
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6525-35
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6525-35
Induction of heat shock protein gp96 by immune cytokines.
Chen YG, Ashok BT, Liu X, Garikapaty VP, Mittelman A, Tiwari RK
Cell stress & chaperones 2003 Fall;8(3):242-8
Cell stress & chaperones 2003 Fall;8(3):242-8
Peptide mimotopes of oncoproteins as therapeutic agents in breast cancer.
Ashok BT, David L, Chen YG, Garikapaty VP, Chander B, Kanduc D, Mittelman A, Tiwari RK
International journal of molecular medicine 2003 Apr;11(4):465-71
International journal of molecular medicine 2003 Apr;11(4):465-71
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of A) human HeLa and B) mouse NIH3T3 cell lysate with GRP94 antibody (clone 9G10.F8.2). Expected molecular weight: 94~96kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin-fixed, paraffin-embedded human breast carcinoma stained with GRP94 antibody (9G10.F8.2).