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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
- Flow cytometry [1]
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- Product number
- NBP1-04346 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-04346, RRID:AB_1521105
- Product name
- Mouse Monoclonal gp96/HSP90B1/GRP94 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteomic Characterization of Epithelial-Like Extracellular Vesicles in Advanced Endometrial Cancer.
Mariscal J, Fernandez-Puente P, Calamia V, Abalo A, Santacana M, Matias-Guiu X, Lopez-Lopez R, Gil-Moreno A, Alonso-Alconada L, Abal M
Journal of proteome research 2019 Mar 1;18(3):1043-1053
Journal of proteome research 2019 Mar 1;18(3):1043-1053
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - PAGE, transferred to PVDF membrane and probed with anti-human TRA 1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: gp96/HSP90B1/GRP94 Antibody (2H3) [NBP1-04346] - Analysis of TRA1 in U87MG cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
