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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- F54810 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- HSP90B1 Antibody / GRP94
- Antibody type
- Polyclonal
- Description
- Purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Vial size
- 0.08 µl, 0.4 µl
- Concentration
- In 1X PBS, pH 7.4, with 0.09% sodium azide
- Storage
- Aliquot the HSP90B1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human HepG2, 2) mouse liver, 3) hamster CHO, 4) human K562 and 5) human HeLa cell lysate with HSP90B1 antibody. Predicted molecular weight ~94 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of human NCI-H460 cells with HSP90B1 antibody (green) and DAPI nuclear stain (blue).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human lung carcinoma tissue with HSP90B1 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human K562 cells with HSP90B1 antibody; Blue=isotype control, Green= HSP90B1 antibody.
