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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- AM09037PU-S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM09037PU-S, RRID:AB_1616599
- Product name
- anti Endoplasmin / HSP90B1 / TRA1 (676-803)
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human TRA1 (676-803 aa) purified from E. coli
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2H3
- Vial size
- 50 µl
- Concentration
- 1.0 mg/ml
Submitted references TAS-116, a Highly Selective Inhibitor of Heat Shock Protein 90α and β, Demonstrates Potent Antitumor Activity and Minimal Ocular Toxicity in Preclinical Models.
Ohkubo S, Kodama Y, Muraoka H, Hitotsumachi H, Yoshimura C, Kitade M, Hashimoto A, Ito K, Gomori A, Takahashi K, Shibata Y, Kanoh A, Yonekura K
Molecular cancer therapeutics 2015 Jan;14(1):14-22
Molecular cancer therapeutics 2015 Jan;14(1):14-22
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis: The cell lysates of hES (20 ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with AM09037PU-N HSP90B1 antibody (1/500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence staining of human ES cell colony with monoclonal anti-human TRA1 antibody (2H3).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Breast at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Heart at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry: AM09037PU-N HSP90B1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 5 µg/ml followed by biotinylated anti-Mouse IgG secondary antibody, Alkaline Phosphatase-Streptavidin and chromogen.
