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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA003901 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA003901, RRID:AB_1851181
- Product name
- Anti-HSP90B1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human HSP90B1, Gene description: heat shock protein 90kDa beta (Grp94), member 1, Alternative Gene Names: GP96, GRP94, TRA1, Validated applications: ICC, WB, IHC, Uniprot ID: P14625, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.05 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Phosphorylated tau interactome in the human Alzheimer’s disease brain
Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis
Proteomic Analyses Reveal High Expression of Decorin and Endoplasmin (HSP90B1) Are Associated with Breast Cancer Metastasis and Decreased Survival
Drummond E, Pires G, MacMurray C, Askenazi M, Nayak S, Bourdon M, Safar J, Ueberheide B, Wisniewski T
Brain 2020;143(9):2803-2817
Brain 2020;143(9):2803-2817
Novel application for pseudopodia proteomics using excimer laser ablation and two-dimensional difference gel electrophoresis
Ito A, Mimae T, Yamamoto Y, Hagiyama M, Nakanishi J, Ito M, Hosokawa Y, Okada M, Murakami Y, Kondo T
Laboratory Investigation 2012;92(9):1374-1385
Laboratory Investigation 2012;92(9):1374-1385
Proteomic Analyses Reveal High Expression of Decorin and Endoplasmin (HSP90B1) Are Associated with Breast Cancer Metastasis and Decreased Survival
Aziz S, Cawthorn T, Moreno J, Dharsee M, Tran-Thanh D, Ackloo S, Zhu P, Sardana G, Chen J, Kupchak P, Jacks L, Miller N, Youngson B, Iakovlev V, Guidos C, Vallis K, Evans K, McCready D, Leong W, Done S
PLoS ONE 2012;7(2):e30992
PLoS ONE 2012;7(2):e30992
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-HSP90B1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA003901 antibody. Corresponding HSP90B1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
