H00001633-M08

antibody from Abnova Corporation

Targeting: DCK

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001633-M08

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001633-M08, RRID:AB_622297

Product name

DCK monoclonal antibody (M08), clone 1D12

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant DCK.

Antigen sequence

WMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRN
EEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQE
VPILTLDVNEDFKDKYESLVEKVKEFLSTL


Isotype

IgG

Antibody clone number

1D12

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The role of HuR in gemcitabine efficacy in pancreatic cancer: HuR Up-regulates the expression of the gemcitabine metabolizing enzyme deoxycytidine kinase.

Costantino CL, Witkiewicz AK, Kuwano Y, Cozzitorto JA, Kennedy EP, Dasgupta A, Keen JC, Yeo CJ, Gorospe M, Brody JR
Cancer research 2009 Jun 1;69(11):4567-72

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged DCK is approximately 3ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to DCK on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol