Explore
Validate
Learn500-P226-100UG
antibody from Invitrogen Antibodies
Targeting: PGF
D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 500-P226-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PlGF-1 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MLPAVPPQQW ALSAGNGSSE VEVVPFQEVW GRSYCRALER LVDVVSEYPS EVEHMFSPSC VSLLRCTGCC GDENLHCVPV ETANVTMQLL KIRSGDRPSY VELTFSQHVR CECRPLREKM KPERCGDAVP RR.
- Concentration
- 0.1-1.0 mg/mL
Submitted references Local delivery of VEGF and SDF enhances endothelial progenitor cell recruitment and resultant recovery from ischemia.
Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release.
Anderson EM, Kwee BJ, Lewin SA, Raimondo T, Mehta M, Mooney DJ
Tissue engineering. Part A 2015 Apr;21(7-8):1217-27
Tissue engineering. Part A 2015 Apr;21(7-8):1217-27
Cyclic stretch induces PlGF expression in bronchial airway epithelial cells via nitric oxide release.
Mohammed KA, Nasreen N, Tepper RS, Antony VB
American journal of physiology. Lung cellular and molecular physiology 2007 Feb;292(2):L559-66
American journal of physiology. Lung cellular and molecular physiology 2007 Feb;292(2):L559-66
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human PlGF-1 by Western Blot analysis PlGF-1 Polyclonal Antibody (Product # 500-P226-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PlGF-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human PlGF-1 by Western Blot analysis PlGF-1 Polyclonal Antibody (Product # 500-P226-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human PlGF-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human PlGF-1 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of PlGF-1 Polyclonal Antibody (Product # 500-P226-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech PlGF-1 Polyclonal Antibody, Biotin (Product # 500-P226BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human PlGF-1.
