DM3524
antibody from Acris Antibodies GmbH
Targeting: PGF
D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- DM3524 - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DM3524, RRID:AB_1770606
- Product name
- anti Placenta growth factor / PGF
- Antibody type
- Monoclonal
- Antigen
- Recombinant Human PlGF-2 [Leu19 â Arg170] produced in insect cells (Cat.-No DA3510)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 342/3B10
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western analysis with recombinant Human PlGF-1 and PlGF-2 derived from Insect cells. The monoclonal Mouse anti-Human PlGF antibody (clone 3B10) Cat.-No DM3524 recognizes solely the PlGF-2 isoform.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunofluorescence with anti-Human PlGF antibodies (clones G10 and 3B10): Sf9 insect cells were infected with recombinant Baculovirus for Human PlGF-1 and PlGF-2. Three days after infection the Immunofluorescence protocol was performed.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry with cryo-sections using monoclonal anti-Human PlGF antibody: Right Picture:Clone#342/3B10, Cat#DM3524Left Picture: Control. The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry with cryo-sections using monoclonal anti-Human PlGF antibody: Right Picture:Clone#342/3B10, Cat#DM3524Left Picture: Control without primary antibody (paralle section). Note, that the syncytiothroblast layer is positiv for PlGF but not other fetal cells insite the trophoblast villi.The experiment was performed by PD. Dr. Jörg Wilting, Pädiatrie I, Zentrum für Kinderheilkunde und Jugendmedizin in Göttingen, Germany.