H00006935-M01

antibody from Abnova Corporation

Targeting: ZEB1 AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006935-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006935-M01, RRID:AB_622396

Product name

ZEB1 monoclonal antibody (M01), clone 4C4

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant ZEB1.

Antigen sequence

NIAIPTVTAQLPTIVAIADQNSVPCLRALAANKQT
ILIPQVAYTYSTTVSPAVQEPPLKVIQPNGNQDER
QDTSSEGVSNVEDQNDSDSTPPKKKMRKTE


Isotype

IgG

Antibody clone number

4C4

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The cytokine IL-6 reactivates breast stromal fibroblasts through transcription factor STAT3-dependent up-regulation of the RNA-binding protein AUF1.

Hendrayani SF, Al-Khalaf HH, Aboussekhra A
The Journal of biological chemistry 2014 Nov 7;289(45):30962-76

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged ZEB1 is 3 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of monoclonal antibody to ZEB1 on HeLa cell . [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

U-2 OS cells were stained with ZEB1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).

Validation comment

Immunofluorescence (Circulating Tumor Cell)