Explore
Validate
LearnNBP1-88845
antibody from Novus Biologicals
Targeting: ZEB1
AREB6, BZP, FECD6, NIL-2-A, PPCD3, TCF8, ZEB, Zfhep, Zfhx1a
Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [21]
- Comments [0]
- Validations
- Immunohistochemistry [7]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-88845 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88845, RRID:AB_11038094
- Product name
- Rabbit Polyclonal ZEB1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ZEB1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Metadherin enhances vulnerability of cancer cells to ferroptosis.
High content screening identifies monensin as an EMT-selective cytotoxic compound.
Clinicopathological significance of ZEB-1 and E-cadherin proteins in patients with oral cavity squamous cell carcinoma.
SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150.
EMT-associated factors promote invasive properties of uveal melanoma cells.
ZEB1-associated drug resistance in cancer cells is reversed by the class I HDAC inhibitor mocetinostat.
The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors.
The clinical role of epithelial-mesenchymal transition and stem cell markers in advanced-stage ovarian serous carcinoma effusions.
Stromal contribution to the colorectal cancer transcriptome.
Epithelial-mesenchymal transition induces endoplasmic-reticulum-stress response in human colorectal tumor cells.
CD95 and CD95L promote and protect cancer stem cells.
Transcription factors OVOL1 and OVOL2 induce the mesenchymal to epithelial transition in human cancer.
Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells.
Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions.
Epithelial to mesenchymal transition in murine tracheal allotransplantation: an immunohistochemical observation.
Zeb1 regulates E-cadherin and Epcam (epithelial cell adhesion molecule) expression to control cell behavior in early zebrafish development.
Activation of miR200 by c-Myb depends on ZEB1 expression and miR200 promoter methylation.
The microRNA-200 family targets multiple non-small cell lung cancer prognostic markers in H1299 cells and BEAS-2B cells.
Dual roles of the transcription factor grainyhead-like 2 (GRHL2) in breast cancer.
Overexpression of ZEB1 associated with metastasis and invasion in patients with gastric carcinoma.
Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization.
Bi J, Yang S, Li L, Dai Q, Borcherding N, Wagner BA, Buettner GR, Spitz DR, Leslie KK, Zhang J, Meng X
Cell death & disease 2019 Sep 17;10(10):682
Cell death & disease 2019 Sep 17;10(10):682
High content screening identifies monensin as an EMT-selective cytotoxic compound.
Vanneste M, Huang Q, Li M, Moose D, Zhao L, Stamnes MA, Schultz M, Wu M, Henry MD
Scientific reports 2019 Feb 4;9(1):1200
Scientific reports 2019 Feb 4;9(1):1200
Clinicopathological significance of ZEB-1 and E-cadherin proteins in patients with oral cavity squamous cell carcinoma.
Yao X, Sun S, Zhou X, Zhang Q, Guo W, Zhang L
OncoTargets and therapy 2017;10:781-790
OncoTargets and therapy 2017;10:781-790
SPROUTY-2 represses the epithelial phenotype of colon carcinoma cells via upregulation of ZEB1 mediated by ETS1 and miR-200/miR-150.
Barbáchano A, Fernández-Barral A, Pereira F, Segura MF, Ordóñez-Morán P, Carrillo-de Santa Pau E, González-Sancho JM, Hanniford D, Martínez N, Costales-Carrera A, Real FX, Pálmer HG, Rojas JM, Hernando E, Muñoz A
Oncogene 2016 Jun 9;35(23):2991-3003
Oncogene 2016 Jun 9;35(23):2991-3003
EMT-associated factors promote invasive properties of uveal melanoma cells.
Asnaghi L, Gezgin G, Tripathy A, Handa JT, Merbs SL, van der Velden PA, Jager MJ, Harbour JW, Eberhart CG
Molecular vision 2015;21:919-29
Molecular vision 2015;21:919-29
ZEB1-associated drug resistance in cancer cells is reversed by the class I HDAC inhibitor mocetinostat.
Meidhof S, Brabletz S, Lehmann W, Preca BT, Mock K, Ruh M, Schüler J, Berthold M, Weber A, Burk U, Lübbert M, Puhr M, Culig Z, Wellner U, Keck T, Bronsert P, Küsters S, Hopt UT, Stemmler MP, Brabletz T
EMBO molecular medicine 2015 Jun;7(6):831-47
EMBO molecular medicine 2015 Jun;7(6):831-47
The EMT-activator ZEB1 induces bone metastasis associated genes including BMP-inhibitors.
Mock K, Preca BT, Brummer T, Brabletz S, Stemmler MP, Brabletz T
Oncotarget 2015 Jun 10;6(16):14399-412
Oncotarget 2015 Jun 10;6(16):14399-412
The clinical role of epithelial-mesenchymal transition and stem cell markers in advanced-stage ovarian serous carcinoma effusions.
Davidson B, Holth A, Hellesylt E, Tan TZ, Huang RY, Tropé C, Nesland JM, Thiery JP
Human pathology 2015 Jan;46(1):1-8
Human pathology 2015 Jan;46(1):1-8
Stromal contribution to the colorectal cancer transcriptome.
Isella C, Terrasi A, Bellomo SE, Petti C, Galatola G, Muratore A, Mellano A, Senetta R, Cassenti A, Sonetto C, Inghirami G, Trusolino L, Fekete Z, De Ridder M, Cassoni P, Storme G, Bertotti A, Medico E
Nature genetics 2015 Apr;47(4):312-9
Nature genetics 2015 Apr;47(4):312-9
Epithelial-mesenchymal transition induces endoplasmic-reticulum-stress response in human colorectal tumor cells.
Zeindl-Eberhart E, Brandl L, Liebmann S, Ormanns S, Scheel SK, Brabletz T, Kirchner T, Jung A
PloS one 2014;9(1):e87386
PloS one 2014;9(1):e87386
CD95 and CD95L promote and protect cancer stem cells.
Ceppi P, Hadji A, Kohlhapp FJ, Pattanayak A, Hau A, Liu X, Liu H, Murmann AE, Peter ME
Nature communications 2014 Nov 4;5:5238
Nature communications 2014 Nov 4;5:5238
Transcription factors OVOL1 and OVOL2 induce the mesenchymal to epithelial transition in human cancer.
Roca H, Hernandez J, Weidner S, McEachin RC, Fuller D, Sud S, Schumann T, Wilkinson JE, Zaslavsky A, Li H, Maher CA, Daignault-Newton S, Healy PN, Pienta KJ
PloS one 2013;8(10):e76773
PloS one 2013;8(10):e76773
Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells.
Jakobsen KR, Sørensen E, Brøndum KK, Daugaard TF, Thomsen R, Nielsen AL
Journal of molecular signaling 2013 Sep 1;8(1):9
Journal of molecular signaling 2013 Sep 1;8(1):9
Poor-prognosis colon cancer is defined by a molecularly distinct subtype and develops from serrated precursor lesions.
De Sousa E Melo F, Wang X, Jansen M, Fessler E, Trinh A, de Rooij LP, de Jong JH, de Boer OJ, van Leersum R, Bijlsma MF, Rodermond H, van der Heijden M, van Noesel CJ, Tuynman JB, Dekker E, Markowetz F, Medema JP, Vermeulen L
Nature medicine 2013 May;19(5):614-8
Nature medicine 2013 May;19(5):614-8
Epithelial to mesenchymal transition in murine tracheal allotransplantation: an immunohistochemical observation.
Konoeda C, Koinuma D, Morishita Y, Sano A, Nagayama K, Motomura N, Kakimi K, Miyazono K, Nakajima J, Nicolls MR, Murakawa T
Transplantation proceedings 2013 Jun;45(5):1797-801
Transplantation proceedings 2013 Jun;45(5):1797-801
Zeb1 regulates E-cadherin and Epcam (epithelial cell adhesion molecule) expression to control cell behavior in early zebrafish development.
Vannier C, Mock K, Brabletz T, Driever W
The Journal of biological chemistry 2013 Jun 28;288(26):18643-59
The Journal of biological chemistry 2013 Jun 28;288(26):18643-59
Activation of miR200 by c-Myb depends on ZEB1 expression and miR200 promoter methylation.
Pieraccioli M, Imbastari F, Antonov A, Melino G, Raschellà G
Cell cycle (Georgetown, Tex.) 2013 Jul 15;12(14):2309-20
Cell cycle (Georgetown, Tex.) 2013 Jul 15;12(14):2309-20
The microRNA-200 family targets multiple non-small cell lung cancer prognostic markers in H1299 cells and BEAS-2B cells.
Pacurari M, Addison JB, Bondalapati N, Wan YW, Luo D, Qian Y, Castranova V, Ivanov AV, Guo NL
International journal of oncology 2013 Aug;43(2):548-60
International journal of oncology 2013 Aug;43(2):548-60
Dual roles of the transcription factor grainyhead-like 2 (GRHL2) in breast cancer.
Werner S, Frey S, Riethdorf S, Schulze C, Alawi M, Kling L, Vafaizadeh V, Sauter G, Terracciano L, Schumacher U, Pantel K, Assmann V
The Journal of biological chemistry 2013 Aug 9;288(32):22993-3008
The Journal of biological chemistry 2013 Aug 9;288(32):22993-3008
Overexpression of ZEB1 associated with metastasis and invasion in patients with gastric carcinoma.
Jia B, Liu H, Kong Q, Li B
Molecular and cellular biochemistry 2012 Jul;366(1-2):223-9
Molecular and cellular biochemistry 2012 Jul;366(1-2):223-9
Quantitative analysis of microRNAs in tissue microarrays by in situ hybridization.
Hanna JA, Wimberly H, Kumar S, Slack F, Agarwal S, Rimm DL
BioTechniques 2012 Apr;52(4):235-45
BioTechniques 2012 Apr;52(4):235-45
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human cerebral cortex shows strong nuclear positivity in glial cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Immunohistochemical staining of human endometrium shows strong nuclear positivity in stromal cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human smooth muscle shows strong nuclear positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human glioma shows strong nuclear positivity in tumor cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human liver shows no nuclear positivity in hepatocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Analysis in human cerebral cortex and liver tissues. Corresponding ZEB1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZEB1 Antibody [NBP1-88845] - Staining of human prostate shows strong nuclear positivity in smooth muscle cells.
