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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34729 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ELMO1 Recombinant Rabbit Monoclonal Antibody (JG34-59)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Rat spleen tissue, mouse spleen tissue, Jurkat, SH-SY-5Y.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG34-59
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ELMO1 in mouse spleen tissue and Jurkat cell lysates. Samples were incubated with ELMO1 monoclonal antibody (Product # MA5-34729), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ELMO1 in paraffin-embedded rat spleen tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with ELMO1 monoclonal antibody (Product # MA5-34729), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of ELMO1 in paraffin-embedded mouse spleen tissue. Samples were heat mediated antigen retrieval with sodium citrate buffer (pH6, 20 min), incubated with ELMO1 monoclonal antibody (Product # MA5-34729), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of ELMO1 in SH-SY-5Y cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with ELMO1 monoclonal antibody (Product # MA5-34729) at a dilution of 1:50, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
